Position: Home > Articles > Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid
FOOD SCIENCE
2004,25
(10)
46-49
荧光假单胞菌AR4产2-酮基-D-葡萄糖酸发酵条件的研究
作 者：
孙文敬;杨庆文;黄惠英;郭金权;赵峰梅;刘敬泽
单 位：
河北师范大学生命科学学院;郑州市生物化工厂;山西省生物研究所
关键词：
荧光假单胞菌AR4;2-酮基-D-葡萄糖酸;发酵;抗噬菌体菌株
摘 要：
以淀粉水解糖为碳源,研究了不同氮源、碳氮比、金属离子、接种量以及环境因子(培养基起始pH值、通气量、温度)对抗噬菌体菌株荧光假单胞菌(Pseudomonas fluorescens)AR4产2-酮基-D-葡萄糖酸发酵的影响。结果表明,氮源、碳氮比、培养基的起始pH值、通气量及温度对该菌的产酸均有显著影响。在适宜培养条件下,该菌的摇瓶产酸及发酵转化率分别可达13.5%和90%左右。
译 名：
Studies on the Fermentation Conditions of Pseudomonas fluorescens AR4 Producing 2-Keto-D-gluconic Acid
作 者：
SUN Wen-jing1,2,YANG Qing-wen2,HUANG Hui-ying3,GUO Jin-quan2,ZHAO Feng-mei2,LIU Jing-ze1,*(1.College of Life Science, Hebei Normal University, Shijiazhuang 050016, China;2.Shanxi Institute ofBiology, Taiyuan 030006, China;3.Zhengzhou Biochemical Factory, Zhengzhou 450001, China)
关键词：
Pseudomonas fluorescens AR4;2-keto-D-gluconic acid;fermentation;bacteriophage-resistant mutant
摘 要：
Using saccharide from corn starch hydrolysate as a sole carbon source, the factors (nitrogen sources, carbon-nitrogenratio, metal ions, the volume of inoculation, initial pH value of the medium, aeration rate and temperature) affecting 2-keto-D-gluconic acid fermentation by bacteriophage-resistant mutant Pseudomonas fluorescens AR4 were investigated. The resultsshowed that the production of 2-keto-D-glluconic acid was significantly affected by nitrogen sources, carbon-nitrogen ratio,initial pH value of the medium, aeration rate and temperature. Under the suitable fermentation conditions, the acid yield and theconversion rate was about 13.5% and 90% respectively.
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