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Position: Home > Articles > Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture FOOD SCIENCE 2004,25 (11) 115-117

补料分批培养生产2-酮基-D-葡萄糖酸的研究

作  者:
孙文敬;赵峰梅;杨庆文;郭金权;秦丽;刘敬泽
单  位:
河北师范大学生命科学学院;郑州市生物化工厂;山西省生物研究所
关键词:
2-酮基-D-葡萄糖酸;荧光假单胞菌AR4;补料分批培养
摘  要:
研究了补糖对荧光假单胞菌(Pseudomonas fluorescens)AR4生产2-酮基-D-葡萄糖酸的影响,并在50kL发酵罐上进行了补料分批培养工业应用性试验。研究结果表明,采用补料分批培养方法,能有效提高发酵液中的产物浓度;开始补糖时发酵液中的残糖浓度以3%~6%为宜;补料分批培养方法及AR4菌株可用于2-酮基-D-葡萄糖酸的工业化规模生产中。
译  名:
Studies on the Fermentative Production of 2-Keto-D-gluconic Acid by Pseudomonas fluorescens in Fed-batch Culture
作  者:
SUN Wen-jing1,2,ZHAO Feng-mei2,YANG Qing-wen2,GUO Jin-quan2,QIN Li3,LIU Jing-ze1,* (1.College of Life Science, Hebei Normal University, Shijiazhuang 050016,China;2.Shanxi Institute of Biology, Taiyuan 030006, China;3.Zhengzhou Biochemical Factory, Zhengzhou 450001,China)
关键词:
keto-D-gluconic acid;Pseudomonas fluorescens AR4;fed-batch culture
摘  要:
The effects of feeding glucose on the production of 2-keto-D-gluconic acid by Pseudomonas fluorescens AR4 werestudied, and the application of fed-batch culture in the industrial production was also investigated in a 50kL fermentor. The resultsshowed that the concentration of the product could be increased effectively in fed-batch culture, the suitable concentration ofresidual glucose in the fermentation broth was 3%~6% while beginning to feed glucose, and the culture method and the strainAR4 could be applied in the industrial fermentation process.

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