当前位置: 首页 > 文章 > 垂体ghrelin mRNA和LH mRNA在大鼠发情期和间情期的表达 中国兽医科学 2010 (2) 197-200
Position: Home > Articles > Expressions of ghrelin mRNA and LH mRNA in the pituitary of rats during estrus and diestrus Chinese Veterinary Science 2010 (2) 197-200

垂体ghrelin mRNA和LH mRNA在大鼠发情期和间情期的表达

作  者:
张文龙;童德文
单  位:
西北农林科技大学动物医学院
关键词:
ghrelin;促黄体生成素;发情期;间情期;大鼠
摘  要:
为了探索垂体ghrelin mRNA和LHmRNA在大鼠发情期和间情期的表达特点,将12只未经产SD雌性大鼠随机分为发情期组和间情期2组,每组6只,通过外部观察和阴道涂片相结合的方法确定发情期和间情期。动物宰杀后取垂体,用RT-PCR方法检测ghrelin mRNA和LH mRNA的表达丰度。结果显示,发情期组垂体ghrelin mRNA的表达丰度显著低于间情期组(P<0.01);发情期组垂体LH mRNA的丰度显著高于间情期组(P<0.01)。结果表明,ghrelin可能在垂体水平上参与了对LH分泌的调节。
译  名:
Expressions of ghrelin mRNA and LH mRNA in the pituitary of rats during estrus and diestrus
作  者:
ZHANG Wen-long,TONG De-wen(College of Veterinary Medicine,Northwest Sci-Tech University of Agriculture and Forestry,Yangling 712100,China)
关键词:
ghrelin;LH;estrus;diestrus;rat
摘  要:
To investigate the expressions of ghrelin mRNA and luteinizing hormone(LH) mRNA in the pituitary of rats during the estrus and diestrus,twelve virginal females SD rats were classified into estrus group (n=6) and diestrus group (n=6).Vaginal smears were examined daily to detect the characters of estrum.All rats were humanely killed by decapitation,and samples of pituitary tissue were collected to detect values of ghrelin mRNA and LH mRNA by semiquantitative RT-PCR method.The results showed that the expression of ghrelin mRNA in the pituitary was significantly higher in the diestrus than in the estrus(P<0.01),and the expression of LH mRNA was significantly higher in the estrus than in the diestrus(P<0.01).It could be concluded that ghrelin might play a major role in regulating the secretion of LH.

相似文章

计量
文章访问数: 11
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊