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Position: Home > Articles > Immunohistochemical localization of ghrelin in the reproductive system of adult WanXi white goose 2009 (1) 33-35

成年皖西白鹅生殖系统ghrelin的免疫组化定位

作  者:
方富贵;蒋书东;李梅清;李福宝
单  位:
安徽农业大学动物科技学院
关键词:
皖西白鹅;生殖系统;ghrelin;免疫组化
摘  要:
为了研究皖西白鹅生殖系统内是否有产生Ghrelin细胞分布,应用免疫组化SABC法并结合DAB显色技术,结果:①在卵巢的生长卵泡中,均有ghrelin免疫阳性细胞,尤其在生长卵泡细胞的颗粒层更为明显;闭锁卵泡的卵泡外腺细胞也呈现ghrelin免疫阳性反应;②在输卵管五段的粘膜层中均观察到ghrelin免疫阳性细胞,其中,漏斗部细胞着色最深,数量最多,膨大部细胞反应最微弱,峡部、子宫部和阴道部ghrelin免疫反应介于二者之间,肌层和外膜均未见ghrelin表达。结论:产生Ghrelin的细胞在成年皖西白鹅卵巢和输卵管中均有广泛的分布,揭示Ghrelin可能调节生殖功能。
译  名:
Immunohistochemical localization of ghrelin in the reproductive system of adult WanXi white goose
作  者:
Fang Fugui,Jiang Shudong,Li Meiqing,Li Fubao(Animal Science and Technology,Anhui Agricultural University,130 Changjiang West Road,Hefei,Anhui Province 230036,fafang@ahau.edu.cn)
关键词:
WanXi white goose;reproductive system;ghrelin;immunohistochemistry
摘  要:
To study the distribution of ghrelin-producing cells in reproductive system of WanXi white geese.Methods:The immunohistochemical SABC method and DAB staining technology were adopted.Results:Ghrelin immunopositive cells(ghrelin-ip) were mainly found in the granularl layer of the grow follicles.Atretic follicle showed specific ghrelin immunostaining,especially follicular grand cells.Ghrelin-ip cells were found in the mucous membrane of tuba uterine.Strong and more ghrelin-ip cells were observed in the infundibulum part,weak ghrelin staining cells in the magnum part,but moderate ghrelin staining cells in isthmus part,utera part and vigina part.No immunoreactive cells were found in the tunica muscularis and tunica adventitia.Conclusion:The results suggested that ghrelin-producing cells exist in the ovary and tuba uterine of the adult WanXi white goose,which indicates that ghrelin may regulate the function of the reproduction.

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