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Position: Home > Articles > Construction and Expression of Eukaryotic Expression Vector of Mature Polypeptide of Duck Interferon Alpha Gene Journal of Northeast Agricultural University(English Edition) 2006,13 (2)

Construction and Expression of Eukaryotic Expression Vector of Mature Polypeptide of Duck Interferon Alpha Gene

作  者:
PEI Fucheng;LI Jingpeng;LI Lu;ZHANG Jianguang;REN Gulping
单  位:
;Harbin Pharmaceutical Group Co., Ltd., Second Chinese Medicine Factory, Harbin Heilongjiang 150078, PRC
关键词:
duck;alpha -interferon;eukaryotic expression;sf9
摘  要:
To study biological activities of Duck Interferon Alpha (DuIFN-a ) and prepare antivirus medicine, the eukaryotic expression vector of mature polypeptide of Duck Interferon Alpha (mDuIFN-alpha ) gene was constructed and expressed in insect cell. By means of PCR technique, the mDuIFN-a gene was cloned from pMD-18-duIFN-alpha recombinant. The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing. The mDuIFN-a gene was ligated with the eukaryotic expression vector pMelBacA, then transfected into Sf9 cell line. Recombinant polypeptide was effectively expressed in insect cell and its molecular weight was 34 ku.

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