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Position: Home > Articles > Expression of Duck Interferon Alpha in BL21(DE3)plysS Journal of Northeast Agricultural University(English Edition) 2005,12 (1)

Expression of Duck Interferon Alpha in BL21(DE3)plysS

作  者:
REN Gui-ping;QU Juan-Juan;PEI Fu-cheng;LI Jing-peng;LIU Xiang-yu;LI Lu;WANG Jun-wei
单  位:
;Life Science College, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC
关键词:
duck;of -interferon;clonging;expression;bl21(de3)plyss
摘  要:
To obtain the protein of duck interferon alpha and study its biological activities, the prokaryotic expression vector of DuIFN-alpha was constructed and expressed in BL21 (DE3) plysS. Using PCR technique, the protein gene of DuIFN-alpha was cloned from pMD-18-duIFN-alpha recombinant, The gene was then inserted to pGEM-T vector and identified by restriction endonuclease analysis and sequencing. DuIFN-a was ligated with the prokaryotic expression vector of pET30 a, then transformed into BL21 (DE3) plysS, The best inducing time and IPTG concentration for the expression of this recombinant protein was tested through the expression of the positive recombinant with different time span and different IPTG concentration. Lots of the protein of DuIFN-alpha were expressed in BL21 (DE3)plysS with 1 mmol centre dot L~(-1) IPTG for 4 hours and its molecular weight for 34 000.

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