当前位置: 首页 > 文章 > 薄层及凝胶色谱法测定树莓籽原花青素的平均聚合度 食品工业科技 2013,34 (11) 317-319
Position: Home > Articles > Determination of the mean Degree of Polymerization(mDP) from raspberry seeds by Thin Layer Chromatography(TLC) and Gel Chromatography(GPC) Science and Technology of Food Industry 2013,34 (11) 317-319

薄层及凝胶色谱法测定树莓籽原花青素的平均聚合度

作  者:
张佰清;闫冬雪
单  位:
沈阳农业大学食品学院
关键词:
薄层色谱;HPLC;凝胶色谱;原花青素;树莓籽
摘  要:
在室温条件下,选择硅胶GF254为固定相,V(甲苯):V(丙酮):V(乙酸)=3:4:1为展开体系,15μL为点样量,通过薄层色谱法分离树莓籽原花青素得到三条谱带,由HPLC和凝胶色谱法分析测定,谱带Ⅰ为单体物质,谱带Ⅱ、Ⅲ平均聚合度为2.65、5.11。
译  名:
Determination of the mean Degree of Polymerization(mDP) from raspberry seeds by Thin Layer Chromatography(TLC) and Gel Chromatography(GPC)
作  者:
ZHANG Bai-qing,YAN Dong-xue (Food Science College of Shengyang Agricultural University,Shenyang 110866,China)
关键词:
TLC;HPLC;GPC;proanthocyanidins;raspberry seeds
摘  要:
At room temperature,select silica gel GF254 as the stationary phase,V(toluene):V(acetone):V(acetic acid)=3:4:1 as the developing system,15μL as the amount of sample application,separate raspberry seed proanthocyanidins by TLC and get three spectrums. HPLC was employed to analyze the compose and GPC was used to get the mDP from each spectrum of TLC. The composition of the spectrumⅠwas monomer material. The mDPs of spectrumⅡand Ⅲ were 2. 65 and 5. 11.

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