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Position: Home > Articles > Study on Rooting in vitro of Phellodendron amurense Journal of Anhui Agricultural Sciences 2013,41 (4) 1442-1443

黄檗试管苗生根的研究

作  者:
金玲玲;姚睿;朴炫春;高日
单  位:
延边大学农学院
关键词:
黄檗;生根;组织培养
摘  要:
[目的]探讨黄檗离体培养生根的最佳条件,为建立其再生体系提供理论依据。[方法]以黄檗试管苗为试材,研究IBA浓度、培养基和活性炭浓度对其生根的影响。[结果]IBA浓度为2.0 mg/L时,黄檗试管苗的生根率为64.2%,生根数为3.9,根长2.4 cm,且植株生长健壮;3/4MS培养基是最适合诱导黄檗试管苗生根的基本培养基,地上部生长健壮,地下部生长良好;活性炭对黄檗试管苗生根有明显促进作用,当培养基中加入1.0 g/L活性炭时,黄檗试管苗的生根率达85.1%,生根数达9.3条,根平均长度达3.1 cm。[结论]黄檗试管苗适宜的生根培养基为3/4 MS+2.0 mg/L IBA+1.0 g/L活性炭。
译  名:
Study on Rooting in vitro of Phellodendron amurense
作  者:
JIN Ling-ling et al(Agriculture College,Yanbian University,Yanbian,Jilin 133002)
关键词:
Phellodendron amurense Rupr;Rooting;Tissue culture
摘  要:
[Objective] To explore the best conditions for in vitro culture of Phellodendron amurense so as to lay technical foundation for regeneration system.[Method] The shoots in vitro of Phellodendron amurense Rupr.were used as experimental material to investigate the effect of IBA concentration,MS medium strength,and active charcoal concentration on rooting in vitro.[Result] The results showed that vigorous growth of plantlet with 3.9 roots per explant and 2.4 cm root length was observed in MS medium supplemented with 2.0 mg/L IBA.MS medium strength affected rooting in vitro,3/4 MS promoted rooting and plantlet growth,and the high biomass was determined.The maximum rooting(85.1%) was obtained in the medium supplanted with 1.0 g/L activity charcoal.[Conclusion] 3/4MS+2.0 mg/L IBA +1.0 g/L activity charcoal was suitable for rooting in vitro of Phellodendron amurense.

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