作 者：
王彦伟;徐凤花;阮志勇;宋金龙;王庆;赵斌;ZHAO Bin
单 位：
中国农业科学院农业资源与农业区划研究所;华中农业大学生命科学技术学院;东北农业大学资源与环境学院
关键词：
沼泥;产甲烷古菌;DGGE;Real-Time PCR
摘 要：
利用DGGE及Real-Time PCR技术对不同地区低温沼气池中沼泥行进发酵前期、后期产甲烷古菌群落变化进行了研究。DGGE技术用于分析发酵前后产甲烷古菌优势群落的变化;Real-Time PCR技术用于分析发酵前期、后期样品产甲烷古菌的数量变化。结果表明,沼气低温发酵的过程中,不同沼泥样品发酵前期、后期产甲烷古菌的优势群落差异明显且数量上也存在较大差异;主要变化的产甲烷古菌的优势群落为甲烷粒菌属(Methanocor-pusculum),甲烷八叠球菌属(Methanosarcina),甲烷鬃毛菌属(Methanosaeta)。甲烷八叠球菌属(Methanosarcina)在黑龙江沼泥样品(A1)发酵后期成为优势群落,甲烷鬃毛菌属(Methanosaeta)在山东发酵前期(B1),后期样品(B2)及安徽发酵前期样品(C1)中占有绝对优势。产甲烷古菌的数量介于104～105个.mL-1之间,其中黑龙江样品中产甲烷菌数量最多,而安徽样品中产甲烷菌数量最少。
译 名：
Study on the Methanogenic Archaea Communities in Low Temperature Biogas Digester by Real-Time PCR and DGGE Methods
作 者：
WANG Yan-wei1,XU Feng-hua1,RUAN Zhi-yong2,SONG Jin-long2,WU Jin4,WANG Qing3,ZHAO Bin3 (1.Resources and Environmental College Northeast Agricultural University,Harbin 150030,China;2.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.College of Life Science and Technology,Huazhong Agricultural University,Wuhan 430070,China)
关键词：
biogas sludge;methanogenic archaea;DGGE;Real-Time PCR
摘 要：
The change of the methanogenic archaea communities and the dominant archaea in low temperature biogas digester were investigated by DGGE.The quantity changes of methanogenic archaea were analyzed by Real-Time PCR.The results indicated that there were significant difference in dominant communities and the archaea quantity between earlier stage and later stage of fermentation in the low temperature biogas digester.The Methanocorpusculum,Methanosarcina and Methanosaeta were the dominant population in the digester.Methanosarcina was the dominant population in the later fermentation for Heilongjiang sample,but the Methanosaeta was the dominant in both earlier and later fermentation stage for Shandong sample,and the earlier stage for Anhui sample.The quantity of Methanogenic archaea ranged between 104~105个·mL-1,among which,the Heilongjiang sample had the highest and the Anhui sample had the lowest.
