作 者：
宋金龙;阮志勇;胡国全;姜瑞波;刘小飞;徐凤花
单 位：
农业部沼气科学研究所;中国农业科学院农业资源与农业区划研究所;东北农业大学资源与环境学院
摘 要：
对从北京周边地区户用沼气池中采集的沼泥进行了富集培养及基因组DNA的提取,以古菌和细菌的通用引物PCR扩增16S rDNA基因的V3可变区,结合应用变性梯度凝胶电泳(DGGE)技术分析沼气池中微生物种群基因的多样性,以及富集前后的微生物群落区系变化,并回收优势的DNA片段进行序列分析。结果表明,微生物群落在沼气池的不同层次上呈现出空间分布多样性的差异,经过富集培养后,微生物种群的多样性及特异性发生了很大的差别。序列比对的结果显示,沼气池中的优势古菌菌群为甲烷鬃菌属(Methanosaeta)与甲烷螺菌属(Methanospiril-lum),细菌为梭菌目(Clostridiales)和互养菌属(Syntrophus)。经过富集培养后,古菌以甲烷八叠球菌属(Methanosarcinamazei)为主,细菌除保持了原有的优势菌群外,γ-变形菌纲(Gamma proteobacterium)也占有绝对的优势。
译 名：
Microbial Diversity and Community Composition in Biogas Sludge and Its Enriched Product
作 者：
SONG Jin-long1,RUAN Zhi-yong2,HU Guo-quan3,JIANG Rui-bo2,LIU Xiao-fei2,XU Feng-hua1 (1.Resources and Environmental College Northeast Agricultural University,Harbin 150030,China;2.Institute of Agricultural Resources and Regional Planning,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3.Biogas Institute of Ministry of Agriculture,Chengdu 610041,China)
关键词：
biogas sludge;enrichment culture;microbial diversity;DGGE
摘 要：
Genomic DNA was extracted from enrichment of biogas sludge which was collected from household biogas digesters in surrounding areas of Beijing.V3 variable fragments of 16S rDNA gene were amplified by PCR in combination with DGGE technology to analyze the genetic polymorphism of microbial populations and their variations before and after enrichment,and predominant DNA fragments were recovered for sequencing.The results indicated prominent microbial diversity in different layer of biogas digester,and the microbial diversity and specificity varied significantly after enrichment.Sequence alignment showed that the Methanosaeta,Methanospirillum,Clostridiales and Syntrophus were the predominant populations in biogas digesters.After enrichment,the predominant populations become Methanosarcina mazei,Clostridiales,Syntrophus and Gamma proteobacterium.
