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Position: Home > Articles > Cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by Vitrification and Encapsulation-vitrification Acta Horticulturae Sinica 2008,35 (4) 607-610

怀地黄玻璃化和包埋玻璃化法超低温保存

作  者:
李明军;周娜;刘杰;张晓丽;李萍;张楠
单  位:
河南师范大学生命科学学院
关键词:
怀地黄;带芽茎段;玻璃化;包埋玻璃化;超低温保存
摘  要:
比较了玻璃化与包埋玻璃化超低温保存怀地黄[Rehmannia glutinosa(Gaertn.)Libosch]‘85-5’带芽茎段的效果。结果表明:4℃低温锻炼5d,在添加二甲基亚砜(DMSO)和乙酰胺的培养基中预培养3d,60%PVS2室温装载25min,PVS20℃脱水30min时,玻璃化和包埋玻璃化两种保存方法均得到本试验条件下最佳结果;两种方法相比,玻璃化法的存活率和再生率均较包埋玻璃化法高,前者的存活率(88.92%)和再生率(64.29%)分别是后者的1.38倍和2.41倍。可见,采用超低温保存怀地黄种质资源是可行的,且玻璃化法优于包埋玻璃化法。
译  名:
Cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by Vitrification and Encapsulation-vitrification
作  者:
LI Ming-jun, ZHOU Na, LIU Jie, ZHANG Xiao-li, LI Ping, and ZHANG Nan (College of Life Sciences, Henan Normal University, Xinxiang,Henan 453007, China)
关键词:
Rehmannia glutinosa (Gaertn.) Libosch; stem with bud; vitrification; encapsulation- vitrification; cryopreservation
摘  要:
The cryopreservation methods of Rehmannia glutinosa (Gaertn.) Libosch by vitrification and encapsulation-vitrification were studied. The results showed that: It was optimal cryopreservation condition for the two methods in the experimentation when the materials were trained at 4 ℃ low temperature for 5 days, then pre-cultured on the medium with DMSO and acetamide for 3 days, loaded with 60% PVS2 for 25 min at room temperature and dehydrated with PVS2 for 30 min at 0 ℃. But compared with the materials cyropreserved by the two methods, the survival rate and regeneration rate by vitrification were both higher than those by encapsulation-vitrification. The survival rate (88.92%) and the regeneration rate (64.29%) of the former were 1.38 times and 2.41 times higher than the latter, suggesting cryopreservation of Rehmannia glutinosa (Gaertn.) Libosch by vitrification is applicable to the germplasm conservation.

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