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Position: Home > Articles > Effects of Three Plant Sterols on Activity of Sterol Regulatory Element and Adipogenesis of 3T3L-1 Journal of Anhui Agricultural Sciences 2013 (12) 5183-5185

三种植物甾醇调控SRE元件和3T3-L1细胞脂肪化活性的作用

作  者:
陈彦光;张弦;刘健
单  位:
合肥工业大学生物与食品工程学院
关键词:
植物甾醇;固醇调控元件;HepG2细胞;荧光素酶
摘  要:
[目的]为了研究植物甾醇对脂质代谢的调控作用。[方法]利用所构建的SRE荧光素酶活性测定系统,体外考察了豆甾醇、β-谷甾醇和菜油甾醇3种常见的植物甾醇对SRE元件的调控活性。[结果]植物甾醇能够增强SRE元件活性。植物甾醇也可以增加3T3-L1细胞的脂肪化作用。[结论]植物甾醇可能正调控脂质的合成。
译  名:
Effects of Three Plant Sterols on Activity of Sterol Regulatory Element and Adipogenesis of 3T3L-1
作  者:
CHEN Yan-guang et al(College of Biology and Food Technology,Hefei University of Technology,Hefei,Anhui 230009)
关键词:
Plant sterol;Sterol regulatory element(SRE);HepG2 cells;Luciferase
摘  要:
[Objective] The research aimed to study the regulating effect of plant sterol on lipid metabolism.[Method]Luciferase reporter vector pGL3-basic that contained one or three sterol regulatory element(SRE) was transfected into HepG2,and luciferase activity of three plant sterols(stigmasterol,β-sitosterol and campesterol) was analyzed.[Result] The plant sterols had positive regulator of SRE.And plant sterols could increase adipogenesis of 3T3L-1.[Conclusion] Plant sterols could increase adipogenesis by increasing SRE activity.

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