摘 要：
取孟士德薰衣草叶片、茎段、芽为外植体,设置6组不同激素水平进行组织培养试验,结果显示:以MS+BA 2.0 mg.L-1(单位下同)+NAA 0.1或MS+BA 2.0+IBA 0.15为培养基诱导愈伤组织效果好;茎段的愈伤诱导率高达92.5%;芽的萌动早、愈伤诱导率达80%;叶的诱导效果最差,愈伤诱导率为35%,且极易褐变死亡。愈伤组织在MS+BA 1.0+IBA 0.1培养基上继续培养,形成大量的丛生芽。单个芽在1/2MS+NAA 0.2培养基上进行生根培养,生根率达98%。试验表明芽适合作快速繁殖材料,茎段适合先形成愈伤组织再诱导植株再生。
译 名：
Callus Induction and Plantlet Regeneration of Lavandula angustifolia 'Munstead'
作 者：
ZHANG Yan-ling,GAO Yuan,WANG Wei-peng,YAO Lei (School of Agriculture and Biology,Shanghai Jiaotong University,Shanghai 200240,China)
关键词：
Lavandula angustifolia 'Munstead';callus induction;plantlet regeneration
摘 要：
Using the leaf,the stem and the bud of L.angustifolia Munstead as explants,we carried out the tissue culture experiments with six culture medium using different levels of hormones.It was shown that the induction results of the MS+BA 2.0 mg·L-1(the same unit as below)+NAA 0.1 or MS+ BA 2.0 +IBA 0.15 medium groups were better than the other groups.The induction rate of callus on stem reached as high as 92.5%;The buds bud earlier,and induction rate of callus reached 80%.However,the induction effect of leaf's callus is not good,with the ratio being 35%,and turning brown death easily.Cultivated in MS+ BA 1.0 +IBA 0.1 culture medium sequentially,Subcultures bud formed massively.The rooting rate of these single bud in 1/2MS+NAA 0.2 culture medium reached 98%.These results indicated that the L.angustifolia Munstead's bud is right material for rapid propagation,while the stem should be used for inducing the plantlet regeneration after the callus formed.
