关键词：
军曹鱼幼鱼;饲料;豆粕蛋白;碳稳定同位素;氮稳定同位素;同位素分馏
摘 要：
配制全鱼粉饲料(以鱼粉为蛋白源)、10%豆粕饲料(用豆粕蛋白10%鱼粉)、20%豆粕饲料(用豆粕蛋白20%鱼粉)等3种等氮等能饲料,分别投喂体质量21.34±1.54 g的幼鱼24 d,研究饲料中豆粕蛋白含量对军曹鱼幼鱼碳、氮稳定同位素分馏的影响。结果表明,随着养殖时间的延长,各饲料组军曹鱼δ13C逐渐上升,δ15N逐渐下降,虽然δ13C和δ15N变化速度上有差异,但除20%豆粕饲料组肌肉的δ15N外,其他各饲料组肌肉和全鱼在实验结束时均已与饲料达到同位素平衡;军曹鱼肌肉和全鱼的Δ13C和Δ15N值均随饲料中豆粕蛋白含量的增加而加大,Δ13C从4.19‰增加到5.04‰,Δ15N从0.18‰增加到2.20‰;军曹鱼全鱼和肌肉的Δ13C和Δ15N相似,但在同位素值变化速度上有差异,因此,在进行生态学营养级研究(长时间尺度)时,肌肉可以代表军曹鱼的同位素特征,而在进行生理学研究(短时间尺度)时,肌肉的同位素变化则不能代表军曹鱼的同位素变化。
译 名：
Effects of Diet Soybean Protein Concentration on Carbon and Nitrogen Isotope Fractionation of Juvenile Cobia(Rachycentron canadum L.)
作 者：
ZHANG Ze-zhao;ZHOU Hui;SHI Gang;CHEN Gang;Fisheries College,Guangdong Ocean University;Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal,Regular High Education Institute of Guangdong Province;
关键词：
Rachycentron canadum;;diet;;soybean meal protein;;δ13C;;δ15N;;isotope fractionation
摘 要：
In order to investigate the effects of diet soybean protein concentration on carbon and nitrogen isotope fractionation of juvenile cobia(Rachycentron canadum L.),three isonitrogenous and isocaloric diets were formulated:the protein source in D1 was fish meal,soybean meal protein replaced 10% and 20% fish meal in D2 and D3,and the diets were fed to juvenile cobia(21.34±1.54 g) for 24 days.The results showed that,with the extending of experiment time,the δ13C of cobia increased and δ15N decreased.Although the changing speed of δ13C and δ15N were different.The whole fish and muscle of cobia fed with different diets had reached isotope equilibrium with their diet when the feeding experiment accomplished,except for D3 muscle.The Δ13C and Δ15N of cobia whole fish and muscle increased with protein concentrationin soybean diet.Δ13C increased from 4.19‰ and 5.04‰,and Δ15N increased from 0.18‰ to 2.20‰.The Δ13C and Δ15N of cobia whole fish and muscle were similar,but the changing speed of cobia whole fish and muscle δ13C and δ15N were different.This indicated that muscle can represent the isotope character of cobia whole fish in trophic level study of ecosystem(long time scale),but can not represent the isotope character of whole fish in physiology study(short time scale).
