作 者：
黄钦成;聂家全;董晓慧;谭北平;迟淑艳;杨奇慧;刘泓宇;章双;杨原志;张海涛
关键词：
军曹鱼幼鱼;钴;生长性能;血液学指标;钴沉积;生物学效价
摘 要：
本试验旨在探究在氯化钴(Co-Cl)和蛋氨酸钴(Co-Met) 2种钴(Co)源下饲料Co含量对军曹鱼幼鱼生长性能、血液学指标和组织Co沉积的影响,并比较2种Co源的生物学效价。以脱维酪蛋白和鱼粉为主要蛋白质源配制基础饲料,在基础饲料中分别添加0(对照)、2、4、8、16、32 mg/kg(以Co计)的Co-Cl或Co-M et,配制11种试验饲料(共用对照)。挑选健康的初始体重为(22.18±0.35) g的军曹鱼幼鱼990尾,随机分为11组,每组分配3个网箱(重复),投喂同一种试验饲料,每个网箱养殖30尾鱼,共投喂10周。结果显示:1) 2种Co源下,特定生长率(SGR)、增重率(WGR)均随饲料Co含量的增加先上升后下降。饲料Co含量极显著影响军曹鱼幼鱼的SGR、WGR和饲料系数(FCR)(P<0.01),饲粮Co源和Co含量的交互作用显著影响SGR、WGR、成活率(SR)(P<0.05)。2)饲粮Co源极显著影响红细胞计数(RBC)(P <0.01),饲料Co含量极显著影响RBC、血红蛋白浓度(HGB)、红细胞压积(HCT)(P<0.01),饲料Co源和Co含量的交互作用极显著影响HCT (P<0.01)。3)饲料Co源极显著影响脊椎骨Co含量(P <0.01),饲料Co含量极显著影响脊椎骨和全鱼Co含量(P <0.01),饲料Co源和Co含量的交互作用显著影响脊椎骨和全鱼Co含量(P<0.05)。由此得出,饲料中适宜含量的Co可改善军曹鱼幼鱼的生长性能和血液学指标,提高组织中Co沉积量。以Co-Cl和Co-Met为Co源,饲料Co含量分别为17.75、19.40 mg/kg时军曹鱼幼鱼可获得最大SGR。以SGR、RBC、脊椎骨Co含量为判据,Co-Met的生物学效价分别为Co-Cl的1.47、1.49、1.12倍。
译 名：
Effects of Cobalt Source and Cobalt Content on Growth Performance,Hematological Indexes and Cobalt Accumulation in Tissues of Juvenile Cobia(Rachycentron canadum)
作 者：
HUANG Qincheng;NIE Jiaquan;DONG Xiaohui;TAN Beiping;CHI Shuyan;YANG Qihui;LIU Hongyu;ZHANG Shuang;YANG Yuanzhi;ZHANG Haitao;Laboratory of Aquatic Animal Nutrition and Feed,Fisheries College,Guangdong Ocean University;Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture;
单 位：
HUANG Qincheng%NIE Jiaquan%DONG Xiaohui%TAN Beiping%CHI Shuyan%YANG Qihui%LIU Hongyu%ZHANG Shuang%YANG Yuanzhi%ZHANG Haitao%Laboratory of Aquatic Animal Nutrition and Feed,Fisheries College,Guangdong Ocean University%Key Laboratory of Aquatic,Livestock and Poultry Feed Science and Technology in South China,Ministry of Agriculture
关键词：
juvenile cobia(Rachycentron canadum);;cobalt;;grow th performance;;hematological indexes;;cobalt accumulation;;bioavailability
摘 要：
This experiment was carried out to investigate the effects of dietary cobalt(Co) content on grow th performance,hematological indexes and Co accumulation in tissues of juvenile cobia(Rachycentron canadum)under two Co sources [cobalt chloride(Co-Cl) and cobalt methionine(Co-M et) ],and to compare the bioavailability of Co-Cl and Co-M et.A basal diet was formulated with vitamin-free casein and fish meal as main protein sources,and 11 experimental diets(the control diet was shared) were prepared by adding 0(control),2,4,8,16 and 32 mg/kg Co from Co-Cl and Co-M et,respectively.A total of 990 juvenile cobia with an initial body weight of(22.18±0.35) g were randomly divided into 11 groups with 3 cages(replicates).Each cage had 30 fish,and the fish in 3 cages were fed a kind of experimental diet to saturation for 10 weeks.The results showed as follow s:1) under two Co sources,the specific grow th rate(SGR) and weight gain ratio(WGR) was increased firstly and then decreased with dietary Co content increasing.Dietary Co content extremely significantly affected the WGR,SGR and feed conversion ratio(FCR)(P<0.01),and the interaction of dietary Co source and Co content significantly affected SGR,WGR and survival rate(SR)(P<0.05).2) Dietary Co source extremely significantly affected erythrocyte count(RBC)(P<0.01),dietary Co content extremely significantly affected RBC,hemoglobin concentration(HGB) and hematocrit(HCT)(P<0.01),and the interaction of dietary Co source and Co content extremely significantly affected HCT(P<0.01).3) Dietary Co source extremely significantly affected Co content in vertebra(P<0.01),dietary Co content extremely significantly affected Co content in vertebra and whole body(P<0.01),and the interaction of dietary Co source and Co content significantly affected Co content in vertebra and whole body(P < 0.05).In conclusion,suitable dietary Co content can improve the grow th performance and hematopoietic indexes of juvenile cobia,and enhance the Co accumulation in tissues.Using Co-Cl and Co-M et as Co sources,the highest SGR of juvenile cobia can be obtained at the Co content of 17.75 and 19.40 mg/kg,respectively.Based on SGR,RBC and Co content in vertebra,the bioavailability of Co-M et is 1.47,1.49 and 1.12 times that of Co-Cl.
