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当前位置: 首页 > 文章 > 响应面法优化常春油麻藤总黄酮含量提取工艺及其生物活性评价 福建农业学报 2018 (11) 1212-1218
Position: Home > Articles > Content and Bioactivity of Flavonoids Extracted from Mucunae sempervirens and Extraction Process Optimization Fujian Journal of Agricultural Sciences 2018 (11) 1212-1218

响应面法优化常春油麻藤总黄酮含量提取工艺及其生物活性评价

作  者：
林志銮;金晓怀;张传海;李宝银;华伟平
单  位：
武夷学院玉山健康管理学院;武夷学院分析测试中心;福建师范大学;福建省生态产业绿色技术重点实验室
关键词：
常春油麻藤;总黄酮;响应面法;羟基自由基;淬灭
摘  要：
通过超声波辅助提取常春油麻藤总黄酮,在单因素试验基础上,利用响应面法优化常春油麻藤总黄酮提取工艺,并对羟基自由基清除能力和牛血清白蛋白结合效应进行评价。结果表明:当料液比1∶25(g·mL-1)、甲醇体积分数60%、超声时间20min、超声功率420W,总黄酮含量预测值为3.64mg·g-1,验证值为3.58mg·g-1,与理论值的相对误差为1.64%,表明该优化方法合理可行。常春油麻藤总黄酮提取液对羟基自由基最大清除率可达85.35%;对牛血清白蛋白具有一定的荧光淬灭作用,淬灭方程为Y=-233.9ln(X)+25.665,R2=0.9906。
译  名：
Content and Bioactivity of Flavonoids Extracted from Mucunae sempervirens and Extraction Process Optimization
作  者：
LIN Zhi-luan;JIN Xiao-huai;ZHANG Chuan-hai;LI Bao-yin;HUA Wei-ping;Wuyi University Analysis and Testing Center;Yushan Health Management College,Wuyi University;Fujian Provincial Key Laboratory of Eco-Industrial Green Technology;Fujian Normal University;
单  位：
LIN Zhi-luan%JIN Xiao-huai%ZHANG Chuan-hai%LI Bao-yin%HUA Wei-ping%Wuyi University Analysis and Testing Center%Yushan Health Management College,Wuyi University%Fujian Provincial Key Laboratory of Eco-Industrial Green Technology%Fujian Normal University
关键词：
Mucunae sempervirens;;total flavonoids;;response surface methodology;;hydroxyl radical scavenging;;quenching
摘  要：
An ultrasonic extraction of flavonoids from Mucunae sempervirens was optimized using a single factor response surface methodology.The content,hydroxyl radical scavenging capacity and bovine serum albumin(BSA)combining ability of the resulting extract were determined for the evaluation.The results showed the optimized conditions to include a methanol volume fraction of 60% and a solid-liquid ratio of 1∶25(g·mL-1)with 420 W ultrasound applied for 20 min to reach a total extracted flavonoid content of 3.64mg·g-1.In comparison to the verification content of 3.58mg·g-1,the difference between the theoretical and experimental values was 1.64%indicating that a reasonable and feasible result had been reached.The hydroxyl radical scavenging rate of the extract was 85.35%,and the fluorescence quenching effect of the extract on the BSA combination test followed the exponential function of Y=-233.9ln(X)+ 25.665(R2=0.9906).

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