中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 沃尔巴克氏体感染对黑腹果蝇性腺和早期胚胎DNA 6mA去甲基化酶基因DMAD表达的影响 昆虫学报 2017 (11) 1292-1299
Position: Home > Articles > Effects of Wolbachia infection on the expression of DNA 6mA demethylase gene DMAD in gonads and early embryos of Drosophila melanogaster Acta Entomologica Sinica 2017 (11) 1292-1299

沃尔巴克氏体感染对黑腹果蝇性腺和早期胚胎DNA 6mA去甲基化酶基因DMAD表达的影响

作  者：
张维;郑雅;王玉凤
单  位：
华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室
关键词：
黑腹果蝇;沃尔巴克氏体;6mA甲基化;DMAD;精巢;卵巢;胚胎
摘  要：
【目的】DNA甲基化是基因修饰的一种重要方式,主要可以形成5-甲基胞嘧啶(5m C)和6-甲基腺嘌呤(6m A)等。目前关于5m C的研究比较多,而关于6m A在真核生物中的研究则较少。沃尔巴克氏体Wolbachia是昆虫中最常见的共生菌之一,可通过多种方式操纵宿主生殖,其中最常见的就是引起精卵细胞质不亲和(cytoplasmic incompatibility,CI),即感染Wolbachia的雄性与未感染的雌性宿主交配后,胚胎致死,但其机制还不清楚。本研究拟从6m A甲基化的角度,探讨Wolbachia影响果蝇生殖的分子机制。【方法】以模式生物黑腹果蝇Drosophila melanogaster为材料,通过实时荧光定量PCR方法,检测Wolbachia感染对果蝇精巢、卵巢以及3个交配组[TT(对照,父母本都未感染),TW(父本未感染,母本感染,胚胎感染,可发育)和WT(CI,致死)]早期胚胎中DNA 6m A去甲基化酶基因DMAD的表达变化。【结果】Wolbachia感染可显著上调1日龄果蝇精巢中DMAD的表达水平,而对卵巢中DMAD的表达没有显著影响。在产卵后0.5 h(中囊胚过渡前)的胚胎中,CI胚胎的DMAD表达水平极显著高于可正常发育的胚胎(TT和TW);在3 h的胚胎(中囊胚过渡期)中,TW和CI胚胎中DMAD的表达量都极显著高于对照组;在6 h的胚胎(中囊胚过渡后)中,CI胚胎中DMAD的表达量相对最低。【结论】Wolbachia感染可能通过干扰宿主果蝇精巢中6m A甲基化水平对精子产生修饰,导致其与正常未感染的卵子受精后胚胎致死。
译  名：
Effects of Wolbachia infection on the expression of DNA 6mA demethylase gene DMAD in gonads and early embryos of Drosophila melanogaster
作  者：
ZHANG Wei;ZHENG Ya;WANG Yu-Feng;School of Life Sciences,Hubei Key Laboratory of Genetic Regulation and Integrative Biology,Central China Normal University;
关键词：
Drosophila melanogaster;;Wolbachia;;6mA modification;;DMAD;;testis;;ovary;;embryo
摘  要：
【Aim】DNA methylation is one of the important ways of gene modification,mainly resulting in5mC and 6mA. At present,there have been many studies related to 5mC,while studies on 6mA in eukaryotes are rare. Wolbachia bacteria are endosymbionts frequently found in insects. They can mediate their hosts' reproduction via several strategies. Cytoplasmic incompatibility( CI) is the most common defect induced by Wolbachia,which arrests the development of embryos from uninfected females that are mated with Wolbachia-infected males. The molecular mechanisms of CI,however,are not clear. The aim of this study is to investigate the mechanisms of Wolbachia affecting the reproduction of Drosophila from the viewpoint of 6mA methylation. 【Methods】The model organism D. melanogaster was used as the test insect. Quantitative RT-PCR was adopted to detect the expression levels of DNA 6mA demethylase gene( DMAD) in testes,ovaries and early embryos of Wolbachia-infected and uninfected D. melanogaster flies. The embryos examined were from three different crosses: TT( the control,with both males and females uninfected),TW( viable,males were uninfected while females were Wolbachia-infected),and WT( lethal CI embryos,with males Wolbachia-infected and females uninfected). 【Results】Wolbachia infection significantly up-regulated the expression of DMAD in testes of 1-day-old flies,but had no effects on DMAD expression in ovaries. In early CI embryos( 0. 5 h,pre-midblastula transition),DMAD expressionin level was significantly higher than those of the control and TW embryos. In the embryos at midblastula transition( 3 h),the transcriptional levels of DMAD in TW and CI were extremely higher than that in the control embryos. In 6 h embryos( post-midblastula transition),however,DMAD expressionin level was the lowest in CI embryos as compared with the control and TW embryos.【Conclusion】Wolbachia infection may significantly change 6mA level in testes of their Drosophila hosts,and thus result in death of early embryos derived from Wolbachia-infected males and uninfected females.

相似文章

导出引用
计量
文章访问数: 8
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46