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Position: Home > Articles > Single nucleotide polymorphisms of the exon 6 of MAOA gene chicken Heilongjiang Animal Science and Veterinary Medicine 2009 (6) 3-4

鸡MAOA基因外显子6的SNP分析

作  者:
刘秀霞;王广增;李岩;周红;刘星亮;韩光;张金成;廖和荣
单  位:
石河子大学动物科技学院
关键词:
吐鲁番斗鸡;新罗曼鸡;单胺氧化酶A基因;PCR-SSCP
摘  要:
研究采用PCR-SSCP方法分析了吐鲁番斗鸡和新罗曼鸡单胺氧化酶A基因外显子6部分序列的多态性。结果表明:吐鲁番斗鸡单胺氧化酶A基因外显子6存在1个单核苷酸多态性(SNP)位点T-102C,产生AA、AB两种基因型;新罗曼鸡未检测到多态,只表现AA一种基因型。x2适合性检验结果表明:2个鸡种均处于Hardy-weinberg平衡状态(P>0.05),说明吐鲁番斗鸡单胺氧化酶A基因外显子6位点基因型频率的分布与新罗曼鸡差异极显著(P<0.01)。
译  名:
Single nucleotide polymorphisms of the exon 6 of MAOA gene chicken
作  者:
LIU Xiu-xia,WANG Guang-zeng,LIAO He-rong,et al(College of Animal Science and Technology,Shihezi University,Shihezi 832003,China)
关键词:
Turpan cockfighting;Xinluoman;MAOA gene;PCR-SSCP
摘  要:
Polymorphism in Exon 6 of MAOA gene of Turpan cockfighting and Xinluoman was analyzed by PCR-SSCP.The result showed that one SNP site was detected in Turpan cockfighting which is T-102C and there two kinds of genotypes(AA and AB).While in Xinluoman there is no SNP site and there is a genotype(AA).The results of x2 test for goodness-of-fit showed that the frequencies of MAOA gene fit with Hardy-weinberg equilibrium in two breeds.The results of x2 test for independence indicates that the genotype frequency of Turpan cockfighting showed significant difference with xinluoman(P<0.01).

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