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Position: Home > Articles > Preparation of inactivated whole-cell and cell chemical components of Aeromonas from Trinyx sinensis Journal of Fisheries of China 2000,24 (2) 167-170

中华鳖气单胞菌菌细胞苗和菌化学成份疫苗研制

作  者:
叶巧真;何建国;翁少萍;邱德全;张邦杰;叶普仁;梁仁杰;潘雷;王晓斌;李本旺;李春枝
单  位:
广东省?;广东省东莞市水产研究所;中山大学生命科学学院
关键词:
中华鳖;嗜水气单胞菌;温和气单胞菌;疫苗
摘  要:
利用分离自中华鳖的嗜水气单胞菌Ah96 10 0 4、Ah970 5 16与温和气单胞菌As970 5 10菌体及其上清制备来活菌细胞苗和化学成份疫苗。肌肉注射中华鳖进行免疫 ,加强免疫 15d后 ,用菌株Ah96 10 0 4腹腔注射进行攻击 ,观察 2 0d。结果表明 ,温和气单胞菌As970 5 10株的菌苗及其胞外分泌物 (ECP)苗组和嗜水气单胞菌Ah96 10 0 4株的外膜成份组对嗜水气单胞菌的攻击的保护率最高 ,分别为 87.5 %和 75 % ,而其它疫苗组的保护率均在 5 0 %以下。
译  名:
Preparation of inactivated whole-cell and cell chemical components of Aeromonas from Trinyx sinensis
作  者:
Ye Qiaozhen,He Jianguo,Weng Shaoping,Qiu Dequan (School of Life Sciences,Zhongshan University, Guangzhou 510275) Zhang Bangjie,Ye Puren,Liang Renjie,Pan Lei,Wang Xiaobin,Li Benwang,Li chunzhi (Dongguan Fisheries Research Institute of Guangdong Provinc
关键词:
Trinyx sinensis,Aeromonas hydrophila,Aeromonas sobria,vaccine
摘  要:
Inactivated whole-cell and cell chemical components were prepared with Aeromonas hydrophila Ah961004,Ah960916,Ah970516 and A.sobria As970510,Soft shell turtles,Trinyx sinensis,were immunized via intramuscular route,after 15d booster doses,challenged with strain Ah961004 intraperitoneally and observed for 20d,The results showed that the group of A.sobria As970510 inactivated whole-cell with its extracellular products and the group of A.hydrophila Ah961004 outer membrane components had highly protection against strain Ah961004, with the protection rate 87.5% and 75% respectively,the other groups were lower than 50%.

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