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Position: Home > Articles > Simultaneous Quantification of Water-soluble Vitamins in Strawberry by High-performance Capillary Electrophoresis Journal of Anhui Agricultural Sciences 2010,38 (24) 12995-12996

草莓中几种水溶性维生素的毛细管电泳测定

作  者:
尹丽;何弘水;王永和
单  位:
漯河医学高等专科学校分析测试实验中心;漯河医学高等专科学校生物化学教研室
关键词:
高效毛细管电泳;水溶性维生素;草莓
摘  要:
[目的]建立高效毛细管电泳同时分离和检测草莓中6种水溶性维生素的方法。[方法]采用高效毛细管电泳方法分离和测定维生素B1、B2、B6、C、PP和叶酸含量,同时研究了缓冲液pH值、电压、检测波长等对测定结果的影响。[结果]实现了6种水溶性维生素的同时分离,回收率为89.9%~97.8%。在柱温20℃,电压20kV,磷酸硼砂缓冲液pH值8.6,进样时间5s,检测波长265nm时,检测效果最好。[结论]建立了毛细管电泳同时测定草莓中6种水溶性维生素含量的方法,并为其他水果维生素的测定提供了依据。
译  名:
Simultaneous Quantification of Water-soluble Vitamins in Strawberry by High-performance Capillary Electrophoresis
作  者:
YIN Li et al (Biochemistry Teaching and Research Section of Luohe Medical College,Luohe,Henan 462002)
关键词:
High-performance capillary electrophoresis;Water-soluble vitamins; Strawberry
摘  要:
[Objective] To establish a method for simultaneously separating and detecting water-soluble vitamins in strawberry. [Method] A total of 6 water-soluble vitamins including VB1,VB2,VB6,VC,nicotinamide and folic acid were quantified by high-performance capillary electrophoresis (HPCE),and the effects of pH value of buffer,voltage and testing wavelength were also studied. [Result] The recovery of six water-soluble vitamins in strawberry is 89.9%-95.8% by HPCE. The optimum conditions were as follows:column temperature 20 ℃,electrophoresis voltage 20 kV,pH value of borate buffer solution 8.6,injection time 5 s,detection wavelength 265 nm. [Conclusion] A method for detecing six water-soluble vitamins simultaneously were established by HPCE.

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