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Position: Home > Articles > Design and Study of Immunoaffinity Chromatography Column for Ofloxacin China Animal Health Inspection 2009,26 (7) 40-42

氧氟沙星免疫亲和色谱柱的研制

作  者:
樊磊;祁克宗;朱良强
单  位:
安徽农业大学;安徽省动物疫病预防与控制中心
关键词:
氧氟沙星;免疫亲和色谱柱;高效毛细管电泳
摘  要:
本试验将氧氟沙星与载体蛋白偶联成人工抗原,用合成的免疫原免疫家兔,制备出高效价抗OFL血清。经纯化后研制出针对OFL特异性吸附的免疫亲和色谱柱,并对色谱柱进行了评价,偶联率为93%,动态柱容量为2204ng/mL,分别添加5mL200ng/mL、300ng/mL和400ng/mL氧氟沙星标准工作液,5mL甲醇洗脱,高效毛细管电泳检测,连续测定6次,测得其回收率为94%、92.67%、95.5%,变异系数为5.32%、3.17%、3.84%。
译  名:
Design and Study of Immunoaffinity Chromatography Column for Ofloxacin
作  者:
Fan Lei1,Qi Kezong1,Zhu Liangqiang2(1.Anhui Agricultural University Anhui Hefei 230036;2.Anhui Provincial Conter for Animal Diseases Control and Prevention 230022)
关键词:
Ofloxacin;Immunoaffinity chromatography column;High performance capillary electrophoresis
摘  要:
In this study,ofloxacin was linked respectively to carrier proteins,and artificial antigens were synthesized successfully.Using the artificial immunogens,rabbits were immunized to obtain the antiserum against ofloxacin.With purified antiserum,the immunoaffinity chromatography column was prepared for ofloxacin.After evaluation of the column,the coupling rate was determined as 93% and the dynamic capacity was 220ng/mL for ofloxacin.Using 5mL methanol as elution buffer,the recoveries were 94 %,92.6 %,95.5 %and C.V.was 5.32%,3.17%,3.84% at levels of 200ng/mL,300ng/mL,400ng/mL in 5mL standard working liquid detected by HPCE for six times.

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