当前位置: 首页 > 文章 > 雪兔Corin基因启动子序列的克隆及与家兔的比较分析 中国养兔杂志 2017 (5) 7-9+23
Position: Home > Articles > Cloning the Promoter of Mountain Hara Corin Gene and Comparing it with Rabbit Chinese Journal of Rabbit Farming 2017 (5) 7-9+23

雪兔Corin基因启动子序列的克隆及与家兔的比较分析

作  者:
李杰;王海禄;郅永伟;尹俊
单  位:
内蒙古农业大学生命科学学院;内蒙古东达獭兔循环产业研究院
关键词:
雪兔;家兔;Corin基因启动子;序列分析
摘  要:
为探明雪兔和家兔Corin基因上的差异,用PCR方法克隆出雪兔Corin基因启动子,并对雪兔、家兔基因启动子序列进行了比对分析。结果显示:成功克隆并拼接出1 841bp的Corin基因启动子序列;雪兔Corin基因启动子序列比家兔相应基因缺失了1 194个碱基;雪兔这段序列有两个CpG岛且家兔相应基因未发现CpG岛;雪兔Corin基因启动子预测到一些家兔的相应位置没有预测到的转录因子,包括EST.TSS和13个Sp1序列。
译  名:
Cloning the Promoter of Mountain Hara Corin Gene and Comparing it with Rabbit
作  者:
LI Jie;WANG Hai-lu;ZHI Yong-wei;YIN Jun;College of Life Science, Inner Mongolia Agricultural University;Inner Mongolia Dongda Rex Rabbit Circulation Industry Research Institute;
单  位:
College of Life Science, Inner Mongolia Agricultural University%Inner Mongolia Dongda Rex Rabbit Circulation Industry Research Institute
关键词:
Lepus timidus;;Rabbit;;Corin gene promoters;;Analysis of gene sequencing
摘  要:
In order to find out the differences between in the Corin gene of lepus timidus and rabbit, a PCRmethod was used to clone the Corin gene promoter of the lepus timidus. The genetic promoter sequence of thelepus timidus and rabbit gene was compared and analyzed. The results showed that the Corin gene promoter se-quence of 1 841 bp was successfully clone; lepus timidus Corin gene promoter sequences corresponding thanthe rabbit is missing 1 194 bases; There are two CpG islands in this sequence and no CpG islands are found inthe corresponding genes of rabbits; Some transcription factors, including a EST.a TSS site, 13 Sp1 sequence,exist in lepus timidus and did not predict in rabbit.

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