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Position: Home > Articles > GEL ELECTROPHORESIS ANALYSIS OF LACTATE DEHYDROGENASE ISOZYMES (LDH) IN THE TISSUES OF SNOW HARE Journal of Northeast Forestry University 1989 (2) 20-26

雪兔组织LDH同工酶电泳分析

作  者：
曾科文;陈凤英;杨明菲;潘雪峰;朱作斌;葛东宁
单  位：
东北林业大学
关键词：
雪兔;LDH同工酶;电泳分析
摘  要：
用不连续系统聚丙烯酰胺凝胶电泳方法,结合热稳定性和尿素抑制作用实验,对雪兔(Lepus timidus Linnaeus)十种组织的LDH同工酶进行电泳分析。实验结果表明,聚丙烯胺凝胶电泳能很好地分离出雪兔组织五种标准LDH同工酶;雪兔LDH_1同工酶相对迁移率为0.50;LDH同工酶对热稳定性程度和抗尿素抑制作用的次序为:LDH_1>LDH_2>LDH_3>LDH_4>LDH_5。这与一般哺乳动物LDH同工酶对热稳定性程度和尿素抑制作用敏感度的次序相一致。雪兔不同组织有其特异性的LDH同工酶电泳图谱;其心脏主要以LDH_1和LDH_2同工酶为主,而股部肌肉主要以LDH_5为主;雪兔肝脏B亚基相对含量为28.85％。并对哺乳动物肝脏同工酶酶谱类型作了初步探讨,认为雪兔肝脏LDH同工酶酶谱类型与草食及躲藏行为有关。
译  名：
GEL ELECTROPHORESIS ANALYSIS OF LACTATE DEHYDROGENASE ISOZYMES (LDH) IN THE TISSUES OF SNOW HARE
作  者：
Zeng Ke wen Chen Feng ying Yang Ming fei Pan Xue feng (North(?)asr Forestry University) Zhu Zuo bin Ge Dong ning (Institute of Zoology, Heilongjiang Academy of Science of Forestry)
关键词：
Lepus timidus;;LDH isozyme;;Cataphoresis;;Analysis
摘  要：
The LDH isozymes in 10 different kinds of tissucs of snow hare were analysed by discontinuous polyacrylamide gel disk electrophoresis, as well as the heat stability experiment and the inhibitory action of urea, the results showed that from the tissues of snow hare, 5 standard bands of LDH isozymes could be isolated clearly by polyacrylamid gel electrophoresis; the relative migration rate of LDH isozymes is 0.50; the degree of heat stability and inhibitory action of urea was in the order of LDH_1>LDH_2>LDH_3>LDH_4>LDH_5. This result was in accordance with that of as LDH isozymes in other kinds of mammals. Different tissues of snow hare had, their specific bands of LDH isozymes The bands of the heart were mainly LDH, and LDH_2 isozymes, but that of septum muscle was LDH_5. The relative content of B subunit in the liver was 28.85%, the banding patterns of the LDH isozymes in liver of different mammals were compared, and it was suggested that the band pattern of LDH isozyrne in liver of the snow hare might be the result of its herbaceous habit of feeding and hiding behavior.

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