当前位置: 首页 > 文章 > 脉冲磁场对枯草芽孢杆菌细胞膜流动性的影响 农业机械学报 2013,44 (11) 202-207
Position: Home > Articles > Effects of the Pulsed Magnetic Field on Membrane Fluidity of Bacillus subtilis Transactions of the Chinese Society for Agricultural Machinery 2013,44 (11) 202-207

脉冲磁场对枯草芽孢杆菌细胞膜流动性的影响

作  者:
钱静亚;马海乐;李树君;刘斌;宋健宇
单  位:
江苏省农产品生物加工与分离工程技术研究中心;江苏大学生物与环境工程学院;江苏大学食品与生物工程学院;中国石油化工股份有限公司;江苏大学江苏省农产品物理加工重点实验室
关键词:
枯草芽孢杆菌;脉冲磁场;细胞膜流动性;荧光偏振
摘  要:
研究了脉冲磁场对枯草芽孢杆菌细胞膜流动性的影响。以DPH为探针,在35℃标记45 min后采用荧光偏振法测定了枯草芽孢杆菌细胞膜的荧光偏振度和荧光各异向性的变化。结果表明,荧光偏振度和荧光各异向性随着磁场强度和脉冲数的增加而增加,2.0 T、2.5 T处理25~30个脉冲,3.0 T处理20~30个脉冲,3.3 T处理10~30个脉冲时细胞膜的荧光偏振度与对照组相比差异显著(P<0.05),2.0 T处理30个脉冲和2.5 T处理25~30个脉冲,3.0 T处理20~30个脉冲,3.3 T处理15~30个脉冲,荧光各异向性与对照组相比差异显著(P<0.05)。由此表明,脉冲磁场可使枯草芽孢杆菌细胞膜流动性下降,而细胞膜流动性的下降会引起细胞膜功能的丧失,这可能是导致细胞死亡的原因。
译  名:
Effects of the Pulsed Magnetic Field on Membrane Fluidity of Bacillus subtilis
作  者:
Qian Jingya;Ma Haile;Li Shujun;Liu Bin;Song Jianyu;Guo Danzhao;School of Food and Biological Engineering,Jiangsu University;Key Lab of Agri-products Physics Processing,Jiangsu University;Chinese Academy of Agricultural Mechanization Sciences;
关键词:
Bacillus subtilis Pulsed magnetic field Cell membrane fluidity Fluorescence polarization
摘  要:
Effects of the pulsed magnetic field on membrane fluidity of Bacillus subtilis were investigated.1,6-diphenyl-1,3,5-hexatriene( DPH) was used as fluorescence probe to label the membrane of B. subtilis at 35℃ for 45 min to determine the changes of fluorescence polarization and fluorescence anisotropy of B. subtilis membrane by fluorescence polarization technique. The results indicated that fluorescence polarization and fluorescence anisotropy increased with the increase of intensity and pulse number of PMF. Compared with the control group,fluorescence polarization increased greatly at 2. 0 T with 25 ~ 30 pulses,2. 5 T with 25 ~ 30 pulses,3. 0 T with 20 ~ 30 pulses and 3. 3 T with 10 ~ 30 pulses( P <0. 05). Fluorescence anisotropy also increased greatly at 2. 0 T with 30 pulses,2. 5 T with 25 ~30pulses,3. 0 T with 20 ~ 30 pulses and 3. 3 T with 15 ~ 30 pulses( P < 0. 05). The membrane fluidity decreased,resulting in the lost of the membrane function,when treated by PMF,which suggested a perhaps mechanisms for the cell death.

相似文章

计量
文章访问数: 7
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊