中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > CDV、CPV、CAV-2及CPIV多重PCR的建立及应用 中国动物检疫 2017,34 (11) 89-93
Position: Home > Articles > Establishment and Rudimentary Application of the Multiplex PCR(mPCR)to the Detection of CDV、CPV、CAV-2 and CPIV China Animal Health Inspection 2017,34 (11) 89-93

CDV、CPV、CAV-2及CPIV多重PCR的建立及应用

作  者：
刘畅;逄春华;刘存;王静;刘琪;梁琳;袁维峰;钱爱东;崔尚金
单  位：
中国农业科学院北京畜牧兽医研究所;青岛机场出入境检验检疫局;吉林农业大学动物科学技术学院
关键词：
多重PCR;犬瘟热病毒;犬细小病毒;犬腺病毒Ⅱ型;犬副流感病毒
摘  要：
为建立一种快速检测犬瘟热病毒(CDV)、犬细小病毒(CPV)、犬腺病毒Ⅱ型(CAV-2)和犬副流感病毒(CPIV)的多重PCR方法,参照Gen Bank中的相关病毒基因序列,分别设计了4对引物,用于特异性扩增CDV H基因、CPV VP2基因、CAV-2 E3基因和CPIV NP基因上的目的片段。通过优化反应条件,建立了同时扩增CDV(410 bp)、CPV(253 bp)、CVA-2(655 bp)和CPIV(868 bp)的多重PCR方法。利用建立的多重PCR方法,对CDV、CPV、CAV-2、CPIV、CDV+CPV+CAV-2+CPIV和犬冠状病毒(CCV)的DNA或c DNA模板进行扩增,发现该方法的特异性良好。利用建立的多重PCR方法与单一PCR方法进行敏感性比较,发现两者敏感性相差10倍,证明多重PCR方法敏感性良好。利用该方法对从北京市采集的30份犬病料样品进行检测,发现CDV阳性率为63.33%(19/30)、CPV阳性率为33.33%(10/30)、CAV-2阳性率为6.66%(2/30)、CPIV阳性率为0(0/30)。检测结果证明建立的多重PCR方法可用于临床诊断。
译  名：
Establishment and Rudimentary Application of the Multiplex PCR(mPCR)to the Detection of CDV、CPV、CAV-2 and CPIV
作  者：
Liu Chang;Pang Chunhua;Liu Cun;Wang Jing;Liu Qi;Liang Lin;Yuang Weifeng;Qian Aidong;Cui Shangjin;College of Animal Science and Technology,Jilin Agricultural University;Institute of Animal Science,Chinese Academy of Agricultural Sciences;Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology,Ministry of Agriculture;Qingdao Airport Entry-exit Inspection and Quarantine Bureau;
单  位：
College of Animal Science and Technology,Jilin Agricultural University%Institute of Animal Science,Chinese Academy of Agricultural Sciences%Beijing Observation Station for Veterinary Drug and Veterinary Biotechnology,Ministry of Agriculture%Qingdao Airport Entry-exit Inspection and Quarantine Bureau
关键词：
multiplex PCR;;Canine distemper virus;;Canine parvovirus;;Canine adenovirus type-2;;Canine parainfluenza virus
摘  要：
In order to establish a multiple PCR method for rapid detection of CDV,CPV,CAV-2 and CPIV,according to the gene sequences in Gen Bank,four pairs of specific primers were designed for amplifying the four specific fragments of CDV H,CPV VP2,CAV-2 E3 and CPIV NP. By optimization of annealing temperature,a multiplex PCR assay of CDV H(410 bp),CPV VP2(253 bp),CAV-2 E3(655 bp)and CPIV NP(868 bp)was established for simultaneous detection of the four viruses. The DNA or c DNA template of CDV,CPV,CAV-2,CPIV,CDV+CPV+CAV-2+ CPIV and CCV were detected by multiplex PCR,and the results showed that the specificity was good. The multiplex PCR method and the single PCR method were compared with the sensitivity. The results of difference was ten times,and the sensitivity of the multiplex PCR was good. Thirty clinical samples of affected dogs from Beijing City were detected by the multiplex PCR,and the positive rate of CDV,CPV,CAV-2 and CPIV were 63.33%(19/30),33.33%(10/30),6.66%(2/30)and 0(0/30),respectively. The results indicated that the established PCR method could be used for clinical diagnosis.

相似文章

导出引用
计量
文章访问数: 10
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46