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Position: Home > Articles > Study on the Degradation Condition of Chitosan by Bromelain Journal of Anhui Agricultural Sciences 2012,40 (34) 16834-16835

菠萝蛋白酶降解壳聚糖条件的研究

作  者:
黄甫;黄利芬;张晗
单  位:
广东海洋大学理学院
关键词:
菠萝蛋白酶;壳聚糖;降解;最适条件
摘  要:
[目的]为菠萝蛋白酶降解壳聚糖制备壳寡糖提供工艺参数和科学依据。[方法]用DNS比色法测定还原糖的含量,探讨不同条件对菠萝蛋白酶降解壳聚糖的影响。用端基分析法测定壳寡糖的分子量,研究菠萝蛋白酶降解壳聚糖过程中壳聚糖分子量的变化。[结果]菠萝蛋白酶降解壳聚糖的最适pH为7.1,最适温度为53℃,最适酶浓度为0.30 g/L。在最适条件下,降解反应进行6 h以上,可以得到平均聚合度为8,平均分子量为1 306的壳寡糖。[结论]菠萝蛋白酶能有效降解壳聚糖,制备壳寡糖。
译  名:
Study on the Degradation Condition of Chitosan by Bromelain
作  者:
HUANG Fu et al(Faculty of Sciences,Guangdong Ocean University,Zhanjiang,Guangdong 524088)
关键词:
Bromelain;Chitosan;Degradation;Optimal condition
摘  要:
[Objective] The aim was to provide scientific basis and process parameters for bromelain degradation of chitosan.[Method] The reducing sugar content was measured by the DNS colorimetric.The effects of different conditions on the degradation of chitosan were investigated.The chitooligosaccharides molecular weight was measured by End-group analysis method.The chitosan molecular weight change was studied in bromelain degradation of chitosan process.[Result] The optimum pH value for degrading chitosan by this enzyme was 7.1 and the optimum temperature was 53 ℃ and the optimum enzyme concentration was 0.30 g/L.After reaction for 6 hours,the chitooligosaccharides which the average degree of polymerization is 8,average molecular weight is 1 306 can be obtained under optimum conditions.[Conclusion] Chitosan can be effectively degraded by bromelain under optimum conditions.

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