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金针菇深层发酵条件及水浴提取菌丝体多糖的研究

作  者:
刘晓鹏;姜宁;向东山;周大寨;郑小江
单  位:
生物资源保护与利用湖北省重点实验室
关键词:
金针菇;深层发酵;多糖;提取
摘  要:
目的:研究金针菇深层发酵的条件、培养菌丝体及菌丝体多糖的提取工艺。方法:通过发酵罐发酵研究金针菇深层发酵的条件及培养菌丝体;通过单因素试验及正交试验研究菌丝体多糖的水浴提取工艺。结果:试验确定了金针菇发酵的最佳种龄为48h、最适接种量是15%,菌丝生长曲线测定确定了发酵周期为培养时间96h,溶氧30%~50%,25℃培养;水浴提取金针菇菌丝体多糖的最佳工艺是:料液比1:60,水浴时间90min,水浴温度60℃。结论:明确了金针菇深层发酵的培养条件,通过发酵罐发酵每升发酵液可得到40g金针菇菌丝体(干重);得到了金针菇菌丝体多糖的水浴提取工艺。
译  名:
Study on Submerged Fermentation Conditions of Flammuling velutipes and Extraction of Mycelial Polysaccharides by Water Bath
作  者:
LIU Xiao-peng1,JIANG Ning1,2,XIANG Dong-shan1,ZHOU Da-zhai1,ZHENG Xiao-jiang1 (1. Hubei Key Laboratory of Biological Resource Conservation and Utilization,Enshi 445000,China; 2. School of Biological Science and Technology,Hubei Institute for Nationalities,Enshi 445000,China)
关键词:
Flammuling velutipes;submerged fermentation;polysaccharides;extraction
摘  要:
The experiment results showed that the optimum conditions of submerged fermentation are as follows: optimal seed age 48 h, inoculation size 15%, culture temperature 25 ℃ and fermentation time 96 h. The optimum conditions for extracting polysaccharides from Flammuling velutipes mycelia by water bath extraction method are as follows: material-liquid ratio 1:60, temperature 60 ℃ and time of 90 minutes.

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