关键词：
聚羟基烷酸;苏丹黑B;红外光谱;气相色谱;假单胞菌属
摘 要：
[目的]获取性状优良的聚羟基烷酸(PHAs)合成菌。[方法]以东湖底泥、山坡裸地土、肥沃菜园土和油脂浸润土为初始样品,经富集、分离纯化、苏丹黑B染色、红外光谱及气相色谱定性定量分析筛选出具PHAs合成能力的细菌菌株。[结果]苏丹黑B染色结果表明,在分离出的12株细菌中有3株细菌具有PHAs合成能力,其编号分别是Zj-5、Zj-8和Zj-9。3株细菌均在1 741 cm-1附近呈现吸收峰,菌株Zj-8和Zj-9的红外谱图相似。菌株Zj-5、Zj-8和Zj-9的PHAs含量分别为146.85、0.65和3.48 mg/L,菌株Zj-5被初步鉴定为假单胞菌属细菌。菌株Zj-5在pH值为5.0~9.0的环境中均能生长,其生长的最适pH值为7.6;在25~35℃下均能正常生长,其最适培养温度和通气量分别为30℃和100 m。l[结论]菌株Zj-5、Zj-8和Zj-9有PHAs合成能力且菌株Zj-5的合成能力最强。
译 名：
Isolation and Identification of Bacterial Polyhydroxyalkanoic Acids Producers and Research on Their Growth Characteristics
作 者：
FENG Jia-ying et al(College of Environmental Technology,Zhejiang Forestry University,Lin'an,Zhejiang 311300)
关键词：
Polyhydroxyalkanoic acids;Sudan black B;Infrared spectrum;Gas chromatogram;Pseudomonas sp.
摘 要：
[Objective] The purpose of the research was to obtain bacterial polyhydroxyalkanoic acids(PHAs) producers with good characters.[Method] With bottom sludge from Donghu Lake,bare soil from hillside,fertile vegetable soil and oil-soaked soil as initial samples,the bacterial strains with ability of synthesizing PHAs were screened out through enriching,isolation and purification,staining with Sudan black B and qualitative and quantitative analyses by infrared spectroscopy and gas chromatography.[Result] The results of staining with Sudan black B showed that there were 3 strains with ability of synthesizing PHAs among the 12 isolated strains and their numbers were Zj-5,Zj-8 and Zj-9 resp.All the 3 strains took on adsorption peaks at the points near 1 741 cm-1 and the infrared spectrums of the strains Zj-8 and Zj-9 were similar.The PHAs contents of the strains Zj-5,Zj-8 and Zj-9 were 146.85,0.65 and 3.48 mg/L and the strain Zj-5 was identified as Pseudomonas sp preliminarily.The strain Zj-5 could grow in the environments with pH value in 5-9 and the optimum pH value for its growth was 7.6;it could grow at the temperature of 25-35 ℃ normally and the optimum culture temperature and ventilation volume for its growth were 30 ℃ and 100 ml resp.[Conclusion] The strains Zj-5,Zj-8 and Zj-9 had ability of synthesizing PHAs and the synthesis ability of the strains Zj-5 was strongest.
