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Position: Home > Articles > A Novel System for Selection of Polyester Biosynthetic Bacteria Journal of Henan Agricultural Sciences 2011,40 (4) 93-95+143

一种快速筛选产聚羟基烷酸细菌方法的建立

作  者:
孙燕飞;王翀;程模香;雷勇辉;熊杰
单  位:
新疆兵团绿洲生态农业重点实验室;新疆出入境检验检疫局;新疆石河子大学生命科学学院
关键词:
菌种筛选;聚羟基烷酸;尼罗蓝;苏丹黑
摘  要:
为了从土壤微生物中快速分离纯化得到产聚β-羟基脂肪酸(PHAs)的微生物菌株,研究比较了几种聚羟基烷酸产生菌筛选方法。结果表明,尼罗蓝染色法优于苏丹黑染色法,而且0.1%的尼罗蓝丙酮染液55℃染色细菌菌落10 min,紫外灯下观察菌落,能合成聚羟基烷酸的菌落具有明显的荧光现象。进而推出对于产生物降解塑料菌株的染色,尼罗蓝染色法优于苏丹黑染色法,尼罗蓝菌落染色法是一种简单、快速、可靠的筛选聚羟基烷酸产生菌的方法。
译  名:
A Novel System for Selection of Polyester Biosynthetic Bacteria
作  者:
SUN Yan-fei1,2,WANG Chong4,CHENG Mo-xiang1,2,LEI Yong-hui2,3,XIONG Jie1,2(1.College of Life & Science,Shihezi University,Shihezi 832003,China;2.Key Laboratory of Oasis EcologyAgriculture of Xinjiang Bingtuan,Shihezi,Xinjiang 832003,China;3.Department of Agronomy Science,Shihezi University,Shihezi 832003,China;4.Xinjiang Exit-entry Inspection and Quarantine Bureau,Urumqi 830063,China)
关键词:
Balteria screening;Polyhydroxybutyrate(PHAs);Nile blue;Sudan black
摘  要:
In order to establish a simple,efficient and reliable method for selecting bacterium strains synthesizing polyhydroxyalkanoic acids(PHAs),several screening methods were compared.The results showed that the method of Nile blue dyeing was better than Sudan black dyeing.When colonies were dyed by Nile blue dissolved in acetone at 0.1% at 55℃ for 10min,and then visualized under UV(365nm),the positive colonies gave fluorescence.Therefore,Nile blue staining method was a better way compared to other screening methods.

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