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当前位置: 首页 > 文章 > 强直性脊柱炎患者外周血CD4+、CD8+ T细胞TCR BV CDR3多态性分析 石河子大学学报(自然科学版) 2016,34 (3) 328-333
Position: Home > Articles > TCR BV CDR3 polymorphism study on CD4+,CD8+ T cells lineage in peripheral blood of ankylosing spondylitis patients Journal of Shihezi University(Natural Science) 2016,34 (3) 328-333

强直性脊柱炎患者外周血CD4+、CD8+ T细胞TCR BV CDR3多态性分析

作  者：
何文丽;余伍忠;徐新玉;王铮;焦敏;邹红云
单  位：
石河子大学医学院;兰州军区乌鲁木齐总医院中医科;兰州军区乌鲁木齐总医院临床医学研究所
关键词：
强直性脊柱炎;TCR;CDR3;BV亚家族
摘  要：
为探讨AS患者外周血CD4+、CD8+T细胞TCR BV CDR3谱型多样性特点,为AS免疫发病机制及治疗提供基础依据。应用RT-PCR方法分别扩增AS患者CD4+、CD8+T细胞TCR BV 26个亚家族CDR3区,采用免疫指纹技术进行TCR BV各家族基因扫描和谱型分析。结果显示,正常健康人CD4+、CD8+TCR BV绝大多数亚家族谱型呈现正态分布(或高斯)分布;AS患者CD4+T细胞绝大多数TCR BV家族呈现与健康对照相似的正态分布,仅少数家族呈不规则异常峰型或偏峰,极少家族出现寡克隆趋势和单克隆,不同患者的CD4+TCR BV家族异常峰型率在4%(1/26)~27%(7/26);AS患者CD8+T细胞,大多数BV家族呈现非正态异常峰型,而少部分家族呈现正态分布的钟型峰图,不同患者CD8+TCR BV家族异常分型率在27%(7/26)~77%(20/26),呈现寡克隆趋势,寡克隆和单克隆峰型的家族数量明显多于CD4+T细胞。由此可知,T细胞在AS的免疫发病机理中扮演重要角色,且提示CD8+T细胞在AS发病机制中起主导作用。
译  名：
TCR BV CDR3 polymorphism study on CD4+,CD8+ T cells lineage in peripheral blood of ankylosing spondylitis patients
作  者：
HE Wenli;YU Wuzhong;XU Xinyu;WANG Zhen;JIAO Min;ZOU Hongyun;School of Medicine, Shihezi University;Urumqi General Hospital of Lanzhou Command of PLA, Institute of Clinical Medical Research;Urumqi General Hospital of Lanzhou Command of PLA, Department of traditional Chinese medicine;
关键词：
ankylosing spondylitis;;T cell receptors;;complementarity determining region3;;BV subfamiles
摘  要：
To study CD4+, CD8+T cells lineage polymorphism of TCR BV CDR3 in peripheral blood of ankylosing spondylitis patients, so as to provide foundation basis to immune pathogenesis research in ankylosing spondylitis. CD4+, CD8+ T cells TCR BV 26 subfamily CDR3 in ankylosing spondylitis patients PBMC were amplified by RT- PCR method, then TCR BV CDR3 lineages polymorphism were analysed by immunization scanning spectrum. Most spectral type of CD4+, CD8+ T cells TCR BV CDR3 in normal controls showed Gauss distribution(or Gauss); Most CD4+T cells TCR BV CDR3 scanning spectrum of ankylosing spondylitis patients showed Gauss distribution(or Gauss) were similar to the normal controls, a few CD4+ T cells TCR BV CDR3 showed skewing peak or a irregular abnormal peak, and a rare CD4+ T cells TCR BV CDR3 showed oligoclonal trend and monoclonal, the rate of different subsets of abnormal peaks was between 4%(1/26) and 27%(7/26) in different patients;Most spectral type of CD8+TCR BV CDR3 showed abnormal distribution peak and a few of it showed Gauss distribution, the rate of different subsets of abnormal peaks was between 27%(7/26)and 77%(20/26) in different patients, the number of abnormal peak was more than CD4+ T cells TCR BV CDR3,including oligoclonal/oligoclonal trend and monoclonal.The study not only further indicates that T cells play an essential role in immune pathogenesis of ankylosing spondylitis, but also suggests CD8+T cells play a dominant role in immune pathogenesis of ankylosing spondylitis.

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