中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 牛地方流行性白血病液相蛋白芯片检测方法的建立 中国兽医科学 2012 (1) 26-30
Position: Home > Articles > Development of a fluorescent microsphere-based assay for the detection of enzootic bovine leukosis Chinese Veterinary Science 2012 (1) 26-30

牛地方流行性白血病液相蛋白芯片检测方法的建立

作  者：
刘志玲;陈茹;舒鼎铭;马静云;朱道中
单  位：
广东出入境检验检疫局;广州出入境检验检疫局;华南农业大学动物科学院
关键词：
牛白血病病毒;gp51基因;xMAP液相蛋白芯片
摘  要：
应用xMAP液相芯片技术原理,以原核表达的重组牛白血病病毒gp51蛋白为抗原,建立了检测牛白血病病毒抗体的LiquiChip液相蛋白芯片检测方法,并进行了特异性、敏感性、重复性与稳定性试验。结果表明,该方法对牛白血病病毒阳性血清检测阳性,对其他常见牛病毒阳性血清检测阴性,表明特异性好;液相蛋白芯片方法对牛白血病病毒抗体的检测敏感性可达119.7ng/μL,对多份牛白血病病毒阳性血清的检测结果显示该方法的检测敏感性与商品化ELISA试剂盒无显著差异;对3份牛白血病病毒阳性血清进行4次重复检测,批内、批间的变异系数均低于10%。所制备的偶联微球芯片保存3个月,其检测结果无显著差异。采用该方法对169份临床牛血清样品进行检测,检出阳性率达57.4%,该检测结果与瑞典进口ELISA试剂盒检测结果的符合率为94.67%。本方法为牛地方流行性白血病的进出境检疫、疫病监测和流行学调查研究提供了一种特异、敏感的新型快速检测技术。
译  名：
Development of a fluorescent microsphere-based assay for the detection of enzootic bovine leukosis
作  者：
LIU Zhi-ling1,CHEN Ru1,SHU Ding-ming2,MA Jing-yun3,ZHU Dao-zhong1,WU Xiao-wei1, LIN Zhi-xiong1(1.Guangdong Entry-Exit Inspection and Quarantine Bureau,Guangzhou 510623,China;2.Institute of Animal Science,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China;3.College of Animal Science,South China Agricultural University,Guangzhou 510642,China)
关键词：
bovine leukemia virus;gp51 gene;xMAP LiquiChip
摘  要：
Recombinant gp51 protein of bovine leukemia virus(BLV) was expressed using a prokaryotic expression system,and purified and covalently coupled to fluorescent xMap microspheres.The purified mysB-gp51 protein was used to establish an xMap method for the detection of antibodies against BLV.And the specificity,sensitivity,reproducibility and stability of the method were examined.For specificity analysis,no positive reaction with positive sera of bluetongue virus,bovine viral diarrhea virus,infectious bovine rhinotracheitis virus,foot-and-mouth disease virus,or bovine ephemeral fever virus was found.For sensiti-vity analysis,as low as 119.7 ng/μL BLV positive serum could be detected and no significant difference with commercial ELISA kit.The variation coefficients of intra-or inter-batch were all less than 10% based on 4 replications of 3 samples.Stability test showed that the coupled-beads could be saved up to 3 months without significant difference.The established method was performed to 169 bovine serum samples with a positive rate of 57.40% which was 94.67% insistent with commercial ELISA kit from Sweden.The results suggested that the BLV xMap assay established in the present study was high specific,being good sensitivity and easy to be manipulated,which provided an available technique for the detection and serological survey of BLV of bovine serum.

相似文章

导出引用
计量
文章访问数: 9
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46