中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 缢蛏丝氨酸蛋白酶基因的序列特征及其表达分析 上海海洋大学学报 2013,22 (4) 481-487
Position: Home > Articles > Molecular characteristics and expression analysis of serine protease from Sinonovacula constricta Journal of Shanghai Ocean University 2013,22 (4) 481-487

缢蛏丝氨酸蛋白酶基因的序列特征及其表达分析

作  者：
金凯;牛东红;王劦;李家乐
单  位：
上海海洋大学农业部水产种质资源重点实验室
关键词：
缢蛏;丝氨酸蛋白酶;序列分析;基因表达
摘  要：
含有clip结构域的丝氨酸蛋白酶是一类新的丝氨酸蛋白酶家族,可能参与非特异性免疫防御功能。从缢蛏(Sinonovacula constricta)cDNA文库中筛选出一条丝氨酸蛋白酶同源EST序列,然后通过5'RACE扩增、测序,拼接得到全长为1 228 bp的cDNA序列,包括66 bp的5'非翻译区和160 bp的3'非翻译区,以及1 002 bp的开放阅读框。阅读框共编码333个氨基酸,含有17个氨基酸的信号肽序列,并含有发卡结构域(clip domain)和胰蛋白酶样丝氨酸蛋白酶结构域(Tryp_SPc domain)。在clip结构域中包含6个半胱氨酸残基形成的3个二硫键,在Tryp_SPc结构域中包含His-Asp-Ser催化三联体(HDS)。该缢蛏丝氨酸蛋白酶基因被命名为ScSP。实时荧光定量PCR(qRT-PCR)分析表明,ScSP在缢蛏的外套膜、水管、鳃、斧足、性腺、肝胰腺6个组织中均有表达,尤其在肝胰腺中表达显著高于其他组织,其次为性腺组织,而水管,外套膜和鳃中表达量最低。缢蛏经鳗弧菌(Vibrio anguillarum)诱导感染后4 h和8 h,肝胰腺中的ScSP基因表达量显著上调。缢蛏丝氨酸蛋白酶的序列特征与表达分析揭示了ScSP是含有clip结构域的丝氨酸蛋白酶基因,参与了非特异性免疫防御,为进一步研究该基因的结构和功能奠定了基础。
译  名：
Molecular characteristics and expression analysis of serine protease from Sinonovacula constricta
作  者：
JIN Kai1,NIU Dong-hong1,WANG Lie1,LI Jia-le1,2(1.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,Ministry of Education,Shanghai Ocean University,Shanghai 201306,China;2.Aquaculture Division E-Institute of Shanghai Universities,Shanghai Ocean University,Shanghai 201306,China)
关键词：
Sinonovacula constricta;serine protease;sequence analysis;gene expression
摘  要：
The serine protease with clip domain is a new serine protease family and plays an important role in innate immunity.One EST sequence with high homology with serine protease gene of other species was found from the cDNA library of Sinonovacula constricta and then the complete ORF and 3' UTR sequence were obtained by PCR.The 5' UTR sequence was got by 5' RACE.The cDNA of this gene was 1 228 bp,which consists of a 66 bp 5' untranslated region(UTR),a 1 002 bp open reading frame(ORF) and a 160 bp 3' UTR.The translated protein is composed of 333 amino acids containing a signal peptide.Sequence analysis of the protein revealed that the protein contained a clip domain and a Tryp_SPc domain.The three disulfide bonds were formed by six conserved cysteines in the clip domain and the catalytic triad(HDS) was contained in the Tyrp_SPc domain.This gene was designated as ScSP.The quantitative reverse transcriptase(qRTPCR) analyses showed that the ScSP can be expressed in six tissues.The expression level of ScSP gene was highest in liver,then in gonad,but lowest in water pipe,mantle and gill.The expression of ScSP gene in liver tissue was up-regulated at 4h and 8h following the challenge with Vibrio anguillarum.These results indicated that ScSP is a new serine protease with clip domain and might be involved in innate immunity,which contributes to understanding the structure and function of serine protease in the future.

相似文章

导出引用
计量
文章访问数: 6
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46