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Position: Home > Articles > Impact of Cd~(2+) on glutathione S-transferase and phosphatase activity in Sinonovacula constricta Progress in Fishery Sciences 2013,34 (2) 119-124

Cd~(2+)对缢蛏谷胱甘肽硫转移酶、磷酸酶活性的影响

作者：

陆慧贤;徐永健

单位：

宁波大学海洋学院应用海洋生物技术教育部重点实验室

关键词：

Cd2+;缢蛏;谷胱甘肽硫转移酶;磷酸酶

摘要：

通过重金属暴露法,研究了不同质量浓度Cd2+(0.005、0.025、0.05、0.1mg/L)在168h内对缢蛏消化腺和鳃组织谷胱甘肽硫转移酶(GST)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)活性的影响。结果显示,Cd2+对两种组织GST活性均有激活作用,且高浓度比低浓度更易快速诱导其活性,但在暴露的后期(>96h),两个较高浓度组(0.05、0.1mg/L)GST活性均出现下降;两种组织的ACP活性(鳃组织0.005mg/L组除外)在暴露的6h即被显著诱导,并表现出剂效关系,但高浓度组更快达到峰值后就持续下降,于暴露中后期(>72h)被不同程度抑制;消化腺(0.005mg/L浓度组除外)和鳃AKP活性在暴露168h内均表现出"抑制-诱导-抑制"的规律,并且较高浓度组活性反而比低浓度组更易被激活,各组AKP活性被诱导达到最高值后就持续下降直至被再次抑制。Cd2+在低浓度水平下即能引起缢蛏两种组织的GST、ACP、AKP活性变化,表明Cd2+的胁迫可对缢蛏机体的解毒体系和新陈代谢产生影响。

译名：

Impact of Cd~(2+) on glutathione S-transferase and phosphatase activity in Sinonovacula constricta

作者：

LU Hui-xian XU Yong-jian* (Key Laboratory of Applied Technology of Marine Biology, Ministry of Education, School of Marine Sciences, Ningbo University, 315211)

关键词：

Cd2+ Sinonovacula constricta Glutathione S-transferase Phosphatase

摘要：

Effect of waterborne Cd2+ at different concentrations (0.005mg/L, 0.025mg/L, 0.05mg/L, and 0.1mg/L) on activities of glutathione S-transferase (GST), acid phosphatase(ACP) and alkaline phosphatase (AKP) in digestive glands and gills of Sinonovacula constricta were studied. It was found that the GST activity in the two tissues was stimulated by Cd2+during the exposure time in all treatments, however, those in higher-dose treatments (0.05 mg/L,0.1 mg/L) decreased with the prolonged exposure. The activation of ACP by Cd2+ in the two tissues began at the 6h time-point, and the activity increased with the increasing concentration of Cd2+.For ACP activity, the higher-dose group reached the peak value earlier than the lower-dose group, and was inhibited after 72h. Except 0.005mg/L dose-treatment in digestive glands, the AKP activities in the two tissues showed a"decrease-increase-decrease"trend during the 168h exposure time in the experiment, and those in higher-dose treatments were stimulated earlier than those in lower ones. AKP activity in each treatment decreased constantly after reaching the peak value respectively. The fluctuations in GST, ACP, and AKP activities in the experiment indicated that exposure to lower concentrations of Cd2+ could have affected the metabolism and detoxification system of the organism.

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Cd~(2+)对缢蛏谷胱甘肽硫转移酶、磷酸酶活性的影响

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