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Position: Home > Articles > Study on polyethlenimine-mediated DNA transfer into A549/DDP lung cancer cells Heilongjiang Animal Science and Veterinary Medicine 2013 (17) 22-24,178

聚乙烯亚胺介导DNA转染肺癌A549/DDP细胞的研究

作  者:
李怀永;邵淑丽;张伟伟;李铁;李玲玲;夏艳;张希锐;贾晓龙
单  位:
齐齐哈尔大学生命科学与农林学院
关键词:
聚乙烯亚胺;转染;A549/DDP细胞
摘  要:
为了确定聚乙烯亚胺(polyethlenimine,PEI)转染肺癌A549/DDP细胞的最佳条件,试验以PEI为载体,将含有绿色荧光蛋白的pRNAT-H1.1/Shuttle-GFP表达载体导入肺癌A549/DDP细胞中,在倒置荧光显微镜下观察不同PEI和DNA质量比、细胞密度、血清含量,以及是否去内毒素和线性化重组质粒对聚乙烯亚胺转染肺癌A549/DDP细胞的影响。结果表明:在质量比为2∶1、肺癌A549/DDP细胞于含10%血清中生长至密度为80%时,转染48 h的转染率最高。
译  名:
Study on polyethlenimine-mediated DNA transfer into A549/DDP lung cancer cells
作  者:
LI Huai - yong;SHAO Shu - li;ZHANG Wei - wei;LI Tie;LI Ling - ling;XIA Yan;ZHANG Xi - rui;JIA Xiao - long;College of Life Science,Agriculture and Forestry,Qiqihar University;
关键词:
polyethlenimine;;transfection;;A549/DDP cells
摘  要:
To investigate the best conditions of polyethylenimine- mediated DNA transfer into A549 /DDP lung cancer cells,the expression vector pRNAT- H1. 1 / Shuttle- GFP containing green fluoreseent protein( GFP) was induced in A549 / DDP lung cancer cells using polyethlenimine( PEI) as a vector. The effects of different PEI / DNA ratios,cell confluence,content of serum,endotoxin and linearization on the transfection efficiencies of PEI were observed using an inverted fluorescence microscope. The results showed that the transfection efficiency of PEI was the highest after 48 h of transfection,if it satisfied the following conditions: PEI / DNA mass ratio was 2∶ 1,the density of A549 / DDP was 10%,and the cell confluence in serum was 80%.

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