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当前位置: 首页 > 文章 > 虫草发酵菌丝体中有效成分麦角甾醇的检测 贵州农业科学 2013,41 (9) 112-115
Position: Home > Articles > Analysis of Free-ergosterol in Fermented Mycelia of Cordyceps by High Performance Liquid Chromatography-Diode Array Detection(HPLC-DAD) Guizhou Agricultural Sciences 2013,41 (9) 112-115

虫草发酵菌丝体中有效成分麦角甾醇的检测

作  者：
李晓飞;陈蓓;张晓杰;孙中华;肖建辉
单  位：
遵义医学院药学院
关键词：
戴氏虫草;江西虫草;发酵菌丝体;麦角甾醇;HPLC-DAD;定量分析
摘  要：
为了建立虫草菌丝体中麦角甾醇的快速定量分析方法。以戴氏虫草、江西虫草的发酵菌丝体为研究材料,采用超声波法提取发酵菌丝体中游离麦角甾醇,利用HPLC-DAD法定量分析麦角甾醇。结果表明,麦角甾醇在0.46~370.00μg/mL有良好的线性关系(R2=0.9979),其检测限和定量限分别为3.50×10-3μg/mL和3.5×10-2μg/mL,灵敏度较高。该方法稳定性、重复性、精密度和准确度良好,其相对标准偏差均在3%以内,尚在HPLC检测允许范围内。戴氏虫草、江西虫草发酵菌丝体中游离麦角甾醇的含量分别为0.165mg/g和0.736mg/g。
译  名：
Analysis of Free-ergosterol in Fermented Mycelia of Cordyceps by High Performance Liquid Chromatography-Diode Array Detection(HPLC-DAD)
作  者：
LI Xiaofei;CHEN Bei;ZHANG Xiaojie;SUN Zhonghua;XIAO Jianhui;School of Pharmaceutical Science,Zunyi Medical College;
关键词：
Cordyceps taii;;Cordyceps jiangxiensis;;fermented mycelia;;ergosterol;;HPLC-DAD;;quantitative analysis
摘  要：
Ergosterol,a major bioactive ingredient of fermented mycelia in C.taii and C.jiangxiensis was extracted by ultrasonic extraction,then a rapid and effective method HPLC-DAD was developed for the quantitative analysis in this study.The calibration curve showed good linearity(R2=0.9979)in the range of 0.46~370.00μg/mL,and the limit of detection(LOD)and limit of quantification(LOQ)were 3.50×10-3 μg/mL and 3.5×10-2 μg/mL,respectively.The method was simple,rapid,and accurate,and with high precision,stability,reproducibility for quantitative determination of ergosterol in fermented mycelia of Cordyceps with less than 3% of relative standard deviation.The content of free-ergosterol was determined in fermented mycelia from two Cordyceps species including C.jiangxiensis and C.taii with this method,and the corresponding content were 0.736and 0.165mg/g,respectively.

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