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Position: Home > Articles > Construction of rFPV (Recombinant Fowlpox Virus) in HA Gene of H9AIV China Animal Health Inspection 2009,26 (3) 31-34

H9亚型禽流感病毒HA基因的重组禽痘病毒构建

作  者:
吴美芹;杨宾宾;孙强强;单虎;李明义
单  位:
广西大学动物科技学院;青岛农业大学动物科技学院;青岛易邦生物工程有限公司
关键词:
禽流感病毒;禽痘病毒;HA基因;重组禽痘病毒
摘  要:
本试验构建了H9亚型禽流感病毒HA基因禽痘病毒转移载体,经转染、蓝斑克隆、筛选和纯化,获得了遗传性状稳定的HA基因重组禽痘病毒。提取感染重组病毒的鸡胚成纤维细胞(CEF)的DNA进行PCR扩增,获得1.7kb的携带有外源目的基因片段。收集纯化的重组病毒CEF细胞,用H9亚型禽流感病毒多克隆血清作一抗,碱性磷酸酶标记的鸡IgG为二抗进行Western blot检测,结果表明重组痘病毒能在体外的CEF细胞表达HA糖蛋白。
译  名:
Construction of rFPV (Recombinant Fowlpox Virus) in HA Gene of H9AIV
作  者:
Wu Meiqin1, Yang Binbin2, Sun Qiangqiang1, Shan Hu1, Li Mingyi3 (1.College of Animal Science and Technology, Qingdao Agricultural University, Shandong Qingdao 266109; 2. College of Animal Science and Technology, Guangxi Agricultural University, Guangxi Nanning 530004; 3. Qing- dao Yebio Bio-engineering Co., Ltd. Shandong Qingdao 266032)
关键词:
AIV; FPV; HA gene; rFPV
摘  要:
In this study, to produce a kind of vaccine including cross-protection in chickens, recombinant fowlpox virus (rFPV) expressing HA9 gene of AIV was constructed. After construction of transfer vector, it was transfected on CEF cells with FPV. Following clonning and purification of blue plaque, the pure HA-fowlpox virus recombinant was obtained, and identified by restriction enzyme analysis and PCR. The result showed that the re-combinant fowlpox virus contained HA gene of AIV and had the stable genetic properties. The result of Western-blot showed that the recombinant fowlpox virus expressed the hemagglutinin in glycoprotein of AIV efficiently.

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