当前位置: 首页 > 文章 > 重组鸡γ-干扰素对H5亚型禽流感灭活苗的免疫增强作用 东北农业大学学报 2010,41 (10) 93-96
Position: Home > Articles > Trial on efficacy of AI H5 type inactivated vaccine enhanced by recombinant chicken interferon-γ Journal of Northeast Agricultural University 2010,41 (10) 93-96

重组鸡γ-干扰素对H5亚型禽流感灭活苗的免疫增强作用

作  者:
曾岩;任晓峰;蔡皓璠;刘明;牛泽;李广兴
单  位:
中国农业科学院哈尔滨兽医研究所;东北农业大学动物医学学院
关键词:
鸡γ-干扰素;禽流感病毒;灭活苗;免疫增强
摘  要:
为研究重组鸡γ-干扰素(Chicken interferonγ,ChIFN-γ)对禽流感灭活疫苗的免疫增强效果,使用重组鸡γ-干扰素作为免疫佐剂,联合禽流感灭活疫苗采用胸部肌肉注射的方式免疫SPF雏鸡,免疫后每周测定血清抗体效价、免疫器官T、B淋巴细胞转化水平,攻毒后计算攻毒保护率。结果表明,鸡γ-干扰素提高了血清抗体水平,免疫器官T、B淋巴细胞转化水平和攻毒保护率,与疫苗组相比多数指标差异显著(P<0.05)。鸡γ-干扰素对H5亚型禽流感灭活疫苗具有免疫增强作用。
译  名:
Trial on efficacy of AI H5 type inactivated vaccine enhanced by recombinant chicken interferon-γ
作  者:
ZENG Yan1,REN Xiaofeng1,CAI Haofan1,LIU Ming2,NIU Ze1,LI Guangxing1(1.College of Veterinary Medicine,Northeast Agricultural University,Harbin 150030,China;2.Harbin Veterinary Research Institute,Chinese Academy of Agricultural Scien-ces,Harbin 150001,China)
关键词:
chicken interferon-γ;avian influenza virus;inactivated vaccine;immunopotentiation
摘  要:
The study aimed to evaluate the adjuvant effect of recombinant chicken interferon γ(ChIFN-γ) on avian influenza virus(AIV) inactivited vaccine.Specific pathogen-free(SPF) chicken were immunized with AIV inactivited vaccine,ChIFN-γor the combination of ChIFN-γand AIV inactivited vaccine.The serum antibody titer and the number of the T/B lymphocytes in chicken were analzyed at a week interval post-immunization.The protection rate of the vaccines to the immunized chicken was calculated after AIV challenge.The results showed that ChIFN-γ had positive effects on the inactivited vaccine,since the adjvant enhanced the titer of serum specific antibody and increased the number of T/B lymphocytes in immunized chicken significantly(P<0.05).ChIFN-γshould be the useful immunore-gulator for avian influenza inactivated vacuine.

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