当前位置: 首页 > 文章 > 广西三黄鸡γ-干扰素基因真核表达载体的构建及表达 西南农业学报 2008,21 (1) 167-169
Position: Home > Articles > Construction and express of recombinant eukaryotic expression vector of Guangxi Sanhuang chicken interferon-gamma Southwest China Journal of Agricultural Sciences 2008,21 (1) 167-169

广西三黄鸡γ-干扰素基因真核表达载体的构建及表达

作  者:
董建宝;谢芝勋;孙建华;刘加波;庞耀珊;邓显文;谢志勤;谢丽基
单  位:
广西兽医研究所
关键词:
鸡γ-干扰素;绿色荧光;真核表达
摘  要:
设计1对特异性引物,采用PCR方法从pMD18-TCHIFN-γ重组载体中扩增得到广西三黄鸡γ-干扰素基因,然后将该基因克隆入含有绿色荧光报告基团EGFP的真核表达载体pEGFP-N1中,构建成pEGFP-N1CHIFN-γ重组质粒,PCR鉴定结果和测序结果均显示,γ-干扰素基因正确地插入到真核表达载体中。使用脂质体转染法将pEGFP-N1CHIFN-γ转染Vero细胞,荧光显微镜下成功地观察到绿色荧光,说明γ-干扰素基因成功表达。
译  名:
Construction and express of recombinant eukaryotic expression vector of Guangxi Sanhuang chicken interferon-gamma
作  者:
DONG Jian-bao,XIE Zhi-xun*,SUN Jian-hua,LIU Jia-bo,PANG Yao-shan,DENG Xian-wen,XIE Zhi-qin,XIE Li-ji(Guangxi Veterinary Institute,Guangxi Nanning 530001,China)
关键词:
chicken interferon-γ;green fluorescence;eukaryotic expression
摘  要:
One pair of specific primers was designed to clone Guangxi Sanhuang chicken interferon-γgene from the recombinant vector pMD18-CHIFN-γ.And then the interferon-γgene was inserted into eukaryotic expression vector pEGFP-N1 which included one EGFP fluorescence gene.The recombinant vecter pEGFP-N1CHIFN-γ was proved correct by PCR and sequencing,then co-transfected into Vero cells,The fluorescence was detected by fluoroscopy,so it was proved that the interferon-γ was expressed successfully.

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