当前位置: 首页 > 文章 > 四氢嘧啶提高中性蛋白酶热稳定性的研究 安徽农业科学 2009,37 (22) 10345-10346
Position: Home > Articles > Study on the Effect of Ectoine on Enhancement of Thermostability of Neutral Protease Journal of Anhui Agricultural Sciences 2009,37 (22) 10345-10346

四氢嘧啶提高中性蛋白酶热稳定性的研究

作  者:
魏胜华;赵永利;余俊;方锦
单  位:
安徽工程科技学院生物化学工程系
关键词:
中性蛋白酶;四氢嘧啶;热稳定性
摘  要:
[目的]提高中性蛋白酶的热稳定性。[方法]在中性蛋白酶酶液中添加四氢嘧啶,考察四氢嘧啶对中性蛋白酶热稳定性的影响。[结果]四氢嘧啶对经热处理的中性蛋白酶有明显的保护作用。添加浓度3 mmol/L的四氢嘧啶可以使经过50℃处理的中性蛋白酶剩余的相对酶活提高12.7%;添加浓度5 mmol/L的四氢嘧啶可以使经过60℃处理的中性蛋白酶剩余的相对酶活提高51.8%。[结论]四氢嘧啶是一种有效的中性蛋白酶热保护剂。
译  名:
Study on the Effect of Ectoine on Enhancement of Thermostability of Neutral Protease
作  者:
WEI Sheng-hua et al(Department of Biochemical Engineering,Anhui University of Technology and Science,Wuhu,Anhui 241000)
关键词:
Neutral protease;Ectoine;Thermostability
摘  要:
[Objective] The aim of the study was to enhance the themostability of neutral protease.[Method] The ectoine was supplemented to the neutral protease solutes to study the effect of ectoine on enhancement of thermostability of neutral protease.[Results] It was demonstrated that ectoine could protect neutral protease from thermal inactivation.The neutral protease with 3mmol/L ectoine was incubated at 50 ℃,the residual enzyme activity of neutral protease was increased by 12.7%;the neutral protease with 5mmol/L ectoine was incubated at 60 ℃,the residual enzyme activity of neutral protease was increased by 51.8% [Conclusions] Ectoine is an effective neutral protease protective reagent.

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