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Position: Home > Articles > 分蘖洋葱鳞茎总黄酮提取工艺及抗氧化活性 Northern Horticulture 2019 (14) 106-116

分蘖洋葱鳞茎总黄酮提取工艺及抗氧化活性

作  者：
黄云彤;娄虎;刘学;崔成日;徐启江
单  位：
哈尔滨艾利姆农业科技有限公司;东北林业大学生命科学学院;黑龙江护理高等专科学校
关键词：
分蘖洋葱;总黄酮;超声提取;响应面法;抗氧化活性
摘  要：
以分蘖洋葱'M-3'鳞茎为试材,用超声波辅助提取黄酮类化合物,在单因素试验基础上,采用Box-Behnken响应面法优化分蘖洋葱总黄酮提取工艺,用DPPH法对总黄酮化合物抗氧化活性进行评价.结果表明:最佳提取工艺参数为料液比1∶45 g·mL-1、乙醇体积分数60%、提取时间40 min、提取温度48℃、超声波功率310 W.在该条件下,分蘖洋葱总黄酮的提取率为3.584%,与模型预测值3.622%相近.分蘖洋葱总黄酮提取物对DPPH·具有一定的清除作用,IC50值为0.179 mg·mL-1.优选的超声波提取工艺提高了分蘖洋葱总黄酮提取率,具有较强的抗氧化活性.
作  者：
HUANG Yuntong;LOU Hu;LIU Xue;CUI Chengri;XU Qijiang;Heilongjiang Nursing College;College of Life Sciences,Northeast Forestry University;Harbin Allium Agricultural Science and Technology Co. Ltd.;
单  位：
HUANG Yuntong%LOU Hu%LIU Xue%CUI Chengri%XU Qijiang%Heilongjiang Nursing College%College of Life Sciences,Northeast Forestry University%Harbin Allium Agricultural Science and Technology Co. Ltd.
关键词：
multiplier onion;;total flavonoids;;ultrasonic extraction;;response surface methodology;;antioxidant activity
摘  要：
Taking the bulbs of 'M-3'multiplier onion(Allium cepaL.var.Aggregatum G.Don)as test material,on the basis of single factor experiment,the optimum ultrasound-assisted extraction process of total flavonoids from bulbs was analyzed by using Box-Behnken design(BBD)with response surface methodology.The antioxidant activity of total flavonoids was evaluated by DPPH method.The results showed that the optimal extraction conditions were determined as follows:1∶40 g·mL~(-1) of solidliquid ratio,60% of ethanol volume fraction,50 ℃ of extraction temperature,300 W of ultrasonic power,30 minutes of extraction time.Under these conditions,the exaction rate of total flavonoids from the bulbs of multiplier onion was 3.586%,which was closed to the model predicted value of 3.622%.The antioxidant tests showed the total flavonoids had a scavenging effect on DPPH·.The IC50 value was 0.179 mg·mL~(-1) which indicated that the flavonoids from multiplier onion bulbs has strong antioxidant activity.The optimal ultrasonic-assisted extraction process improved the extraction rate of total flavonoids from the bulbs of multiplier onion,and the extracts by this process also exhibited a better antioxidant activity.

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