摘 要：
[目的]探讨厚垣孢普可尼亚菌产厚垣孢子的条件。[方法]采用两步培养法测定碳源、氮源、碳氮摩尔比、pH等不同营养环境条件对厚垣孢普可尼亚菌PC021120-152菌株产厚垣孢子的影响。[结果]PC021120-152菌株的最佳产厚垣孢子条件为:初始碳源浓度为3.00 g/L,碳氮摩尔比为2.5∶1,最佳碳氮源组合为蔗糖/NaNO_3,培养基最佳pH为8.0。PC021120-152菌株的最佳产分生孢子条件为:初始碳源浓度为3.00 g/L,碳氮摩尔比为2.5∶1,最佳碳氮源组合为D-果糖/NaNO_3,最佳pH为5.0。[结论]该研究结果可为厚垣孢普可尼亚菌生防制剂的生产调控提供依据。
译 名：
Optimum Conditions for Sporulation of Pochonia chlamydosporia in Two-stage Cultivation
作 者：
SHI Yan;XIAO Shun;LIU Guo-kun;ZHANG Shao-sheng;College of Plant Protection,Fujian Agriculture and Forestry University;
关键词：
Pochonia chlamydosporia;;Sporulation;;Nutritional conditions
摘 要：
[Objective]To discuss the nutritional conditions for sporulation of Pochonia chlamydosporia. [Method]A two-stage cultivation method was used to determine the effects of various nutritional and environmental conditions( carbon sources,nitrogen sources,carbon-nitrogen molar ratio,and p H) on the sporulation of P. chlamydosporia PC021120-152.[Result]Chlamydospore production of PC021120-152 strains was the highest when the initial carbon concentration was 3. 00 g/L; the carbon-nitrogen molar ratio was 2. 5∶ 1; sucrose/NaNO_3 was as the carbon/nitrogen source,and the p H was 8. Conidiophore production of PC021120- 152 strains was the highest when the initial carbon concentration was 3. 00 g /L; the carbon- nitrogen molar ratio was 2. 5∶ 1; D-fructose/NaNO_3 was as the carbon/nitrogen source,and the p H was 5. 0.[Conclusion]The research provides references for industrialization of biocontrol agents of P. chlamydosporia.
