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Study on Genetic Diversity among Soybean in Heilongjiang Province

作  者:
HU Guofu;HU Xiaomei;CHANG Ying;LIU Di;HU Baozhong
单  位:
;College of Life Science, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC
关键词:
soybean;rapd;protein;genetic character
摘  要:
Genetic characters of 10 soybean breeds in Heilongjiang Province were studied using RAPD technique. We calculated the genetic similarity and genetic distance, made the cluster analysis, and also studied the genetic relationship among the 10 breeds. Wefound 93 amplification segments by RAPD analysis, in which there were 70 polymorphism segments, 75.26% of amplification segments, using 13 random primers, 11, 12, 5, 4, 7, 8, 3, 7, 2, 5, 10, 10, 9 amplificated bands were found. There were 7.15 bands perprimer in average. The genetic similarity among the breeds ranged from 0.6848 to 0.9019, and the genetic distance ranged from 0.0981 to 0.3152. Genetic relationship between 90035 and 9674 was the closest, and between Heifeng 27 and 90035 was the farthest. Protein analysis revealed that the closest genetic relationship was between 90035 and 90024, and the farthest was between 1330 and Sui 4, and between 1330 and Heinong 39.

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