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Position: Home > Articles > Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin Journal of Northeast Agricultural University(English Edition) 2006,13 (1)

Screening of Transgenic Soybean Transformed by Means of Pollen-tube Using Kanamycin

作  者:
ZHANG Shuzhen;XU Pengfei;ZHANG Dayong;LIN Shifeng;LI Wenbin;HAN Yingpeng;YANG Chuanping
单  位:
;Soybean Research Institute, Northeast Agricultural University, Harbin Heilongjiang 150030, PRC
关键词:
soybean;pollen-tube method;kanamycin
摘  要:
Kanamycin was used to screen T_0 seeds of the variety Dongnong 46 transformed by means of pollen-tube method. The results showed that 400 mg-L~(-1) kanamycin could inhibit growth of non-transgenic plants, and 2 positive plants were gotten combined with Gus dyeing and PCR detection. It is proved that this method is e-conomic and effective in preliminary screening the transgenic plants.

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