单 位：
长春科技学院医药学院;吉林农业大学中药材学院
关键词：
人参茎叶总皂苷;高效液相色谱法;腐皮镰刀菌;抑制率;抑菌机理
摘 要：
采用HPLC法确定人参茎叶总皂苷中5种单体皂苷的含量;生长速率法测定人参茎叶总皂苷(TGSL)对腐皮镰刀菌菌丝生长的抑制作用;载玻片萌发法测定人参茎叶总皂苷水溶液对腐皮镰刀菌孢子萌发的抑制作用;测定人参茎叶总皂苷处理前后的腐皮镰刀菌电导率变化情况,进一步确定细胞膜透性变化情况;考马斯亮蓝G-250比色法测定腐皮镰刀菌可溶性蛋白质含量的影响;紫外吸收法测定吸光率的变化速度,确定菌体过氧化氢酶的活力。结果表明:人参茎叶总皂苷中人参皂苷Re、Rg1、Rf、Rd、Rb1的含量为1.62%,0.66%,0.10%,1.23%,0.36%;当人参茎叶总皂苷质量浓度为10 mg/m L时,对腐皮镰刀菌菌丝生长抑制率为76.79%,EC50为3.530 2 mg/m L;对孢子萌发的抑制率达81.65%,EC50为3.037 7 mg/m L;人参茎叶总皂苷能引起菌丝细胞膜透性的变化,导致液体培养基电导率增加,菌丝体可溶性蛋白含量降低,过氧化氢酶活性下降;菌丝变细且节间拉长,生长稀疏,菌丝体胞内物质外渗,内含物变少,阻碍腐皮镰刀菌菌丝的生长。
译 名：
Inhibiting Effects of Total Ginsenosides of Ginseng Stems and Leaves against Fusarium solani and Their Antibacterial Mechanism
作 者：
LIU Fangfang;ZHANG Aihua;LEI Fengjie;XU Yonghua;ZHANG Lianxue;Pharmaceutical College,Changchun Science and Technology College;College of Chinese Medicinal Materials,Jilin Agricultural University;
关键词：
total ginsenoside of stem and leaf;;HPLC;;Fusarium solani;;inhibition rate;;antibacterial mechanism
摘 要：
The content of five kinds of monomer saponins in total ginsenosides of ginseng stems and leaves was determined with HPLC. The inhibitory effect of TGSL on F. solani mycelial growth was studied using disc diffusion method. The germination test was used to determine the inhibitory effect of TGSL aqueous solution on spore germination. The changes in conductivity of F. solani with or without TGSL treatment were compared to study cell membrane permeability. The Coomassie G-250 brilliant blue colorimetric method was used to determine the effects of TGSL on the soluble protein content of F. solani. Catalase activity was determined by measuring changes in absorption. Results:The content of ginsenoside Re,Rg1,Rf,Rd and Rb1 were 1. 62%,0. 66%,0. 10%,1. 23% and0. 36%,respectively. We found that 10 mg/m L TGSL had strong inhibitory action on F. solani,with an inhibition rate of 76. 79% and EC50 of 3. 530 2 mg/m L. The inhibition rate of spore germinationreached 81. 65%,and the EC50 was 3. 037 7 mg/mL. TGSL altered the membrane permeability of hyphal cells,decreased soluble protein content and decreased catalase activity. Hyphae grew thinner and internodes elongated,and mycelial intracellular materials extravasated and inclusion became less,hindering the growth of hypha.
