摘 要：
考察小鼠口服人参茎叶总皂苷(GSLS)水溶液(50 mg/L)的抗氧化作用。将30只雌性ICR小鼠随机分成5组,A、B、C和D组小鼠一次腹腔注射环磷酰胺(Cy)诱导氧化应激;E组不注射Cy作为空白对照。A组试验全程每天饮水口服GSLS溶液;B组于Cy应激前每天饮水口服GSLS溶液;C组于Cy应激后每天饮水口服GSLS溶液;D组不口服GSLS,作为Cy应激对照。于试验第9天处死小鼠,检测脏器(胸腺和脾脏)指数以及胸腺、脾脏中丙二醛(MDA)、总超氧化物歧化酶(T-SOD)含量。结果表明,小鼠饮水口服GSLS可显著缓解由Cy诱导的氧化应激作用,提高应激小鼠的胸腺指数及血清中T-SOD活性,脏器中丙二醛MDA水平降低。Cy应激前、后均饮用GSLS的抗应激效果最佳,但应激前与应激后饮用人参茎叶皂苷无显著性差异。
译 名：
Effect of ginseng stem-leaf saponins by oral administration on cyclophosphamideinduced oxidative stress in mice
作 者：
PAN Hang;YU Jia;SHI Yue;MAO Yi-han;HU Song-hua;College of Animal Sciences of Zhejiang University;
关键词：
ginseng stem-leaf saponins;;anti-oxidative stress;;cyclophosphamide
摘 要：
Objective:To investigate the anti-oxidative effects of drinking ginseng stem-leaf saponins(GSLS) solution(50 mg/L) in mice.Methods:Thirty female ICR mice were randomly assigned into five groups with 6 of each.The mice were intraperitoneally(i.p.) injected with cyclophosphamide(Cy) to induce oxidative stress in groups A,B,C and D;and injected with saline solution in group E.The mice in group A were orally administered GSLS in drinking water daily throughout the study;whereas the mice in group B or C were administered GSLS daily before or after the injection of Cy.The mice in group D were not administered GSLS.The animals were weighed and sacrificed 4 d post Cy injection.Blood was collected and thymus and spleen were isolated to analyse the organs indices,total superoxide dismutase(T-SOD) activities,and malondialdehyde(MDA)level.Results and Conclusions:Administration of GSLS in drinking water significantly reduced the Cy-induced oxidative stress in mice.GSLS increased thymus indices,serum T-SOD activities and decreased the level of MDA in the organs.Group A had the best anti-oxidative activities,whereas no significant difference between groups B and C was found.
