作 者：
钟慈平;魏炜;王正虹;邱宏;胡黎黎;顾万江;甘源;唐小琴;李林
单 位：
西南大学食品科学学院;重庆市疾病预防控制中心;西南大学校医院
关键词：
超高压液相色谱;辣椒面;刚果红;含量测定
摘 要：
目的:建立测定辣椒面中刚果红染料含量的超高效液相色谱方法。方法:采用氨水-乙醇(30∶70,V/V)提取剂,超声加热辅助提取3次,合并提取液、浓缩后过HLB固相萃取小柱,以5 mL 1%甲酸-甲醇溶液淋洗、5 mL 10%氨水-甲醇溶液洗脱,收集洗脱液、氮吹浓缩后定容测定。使用BEH C18色谱柱(50 mm×2.1 mm,1.7 μm);流动相:A为乙腈,B为10 mmol/L乙酸铵溶液,梯度洗脱;流速0.4 mL/min;检测波长498 nm;柱温40℃。结果:回收率为72%~96%,方法检出限为0.0290mg/kg,相对标准偏差为2.16%~5.97%(n=6),线性方程相关系数达到0.9999。结论:本方法简便、快速、准确、灵敏,适用辣椒面中刚果红染料含量的测定。
译 名：
Determination of Congo Red in Chili Powder by Solid Phase Extraction Coupled with Ultra-High Performance Liquid Chromatography
作 者：
ZHONG Ci-ping;WEI Wei;WANG Zheng-hong;QIU Hong;HU Li-li;GU Wan-jiang;GAN Yuan;TANG Xiao-qin;LI Lin;College of Food Science, Southwest University;Chongqing Center for Disease Control and Prevention;Hospital of Southwest University;
关键词：
ultra-high performance liquid chromatography (UPLC);; chili powder;; Congo red;; quantitative determination
摘 要：
Objective: To establish a method for determining Congo red in chili powder by ultra-high performance liquid chromatography (UPLC). Methods: Samples were extracted with a mixture of ammonia and ethanol (30:70, V/V). The extraction process was aided by ultrasonic treatment and repeated three times. The combined extract was concentrated and then clean-up on an HLB solid-phase extraction cartridge by washing with 5 mL of 1% formic acid in methanol and elution with 5 mL of 10% ammonia in methanol. The collected eluate was concentrated under a stream of nitrogen prior to analysis. The chromatographic separation was achieved within 4 min on a BEH C18 column (50 mm×2.1 mm, 1.7 μm) by gradient elution using acetonitrile as mobile phase A and 10 mmol/L ammonium acetate as mobile phase B at a flow rate of 0.4 mL/min. The detection wavelength was set at 498 nm and the column temperature was maintained at 40 ℃. Results: Average recoveries of Congo red from chili samples spiked three concentration levels ranged from 72% to 96% with relative standard deviation of 2.16%–5.97% (n = 6). The detection limit of the proposed method was 0.029 0 mg/kg, and the correlation coefficient for the calibration curve was 0.999 9. Conclusion: This method is simple, rapid, accurate, sensitive and suitable for detecting Congo red dye in chili powder.
