中国农业科学院农业信息研究所 中国农学会
Agricultural Information Institute of CAAS China Association of Agricultural Science Societies
En
Ch
中国农业科学院农业信息研究所 中国农学会 主办

AI检索

订阅

返回顶部
当前位置: 首页 > 文章 > 超高压液相色谱快速测定银杏黄酮苷元 中国农学通报 2015,31 (9) 257-260
Position: Home > Articles > Rapid Determination of Flavonol Glycosides in Folium Ginkgo by UPLC-UV Chinese Agricultural Science Bulletin 2015,31 (9) 257-260

超高压液相色谱快速测定银杏黄酮苷元

作  者：
肖强;陈小强;周大寨
单  位：
湖北民族学院/生物资源保护与利用湖北省重点实验室
关键词：
超高压液相色谱;银杏叶;黄酮醇苷
摘  要：
为比较鄂西南不同核用银杏品种叶片中3种主要黄酮苷元含量,笔者采用超高压液相色谱-紫外检测方法,在5 min内实现了银杏叶中3种主要黄酮苷元的快速分离,该方法线性范围为1~12μg/m L,相关系数为0.9999,相对标准偏差小于2.5%。对不同核用银杏品种叶片测定结果表明,在5个核用银杏品种中,不同品种间总黄酮苷元含量具有显著差异,其中以‘恩银2号’含量最高;各品种槲皮素含量均较低,而异鼠李素含量在总苷元中占较高比例。对银杏叶的采收时机,仅仅考察总黄酮苷元是不够的,利用笔者建立的方法可以实现对银杏叶中3种主要黄酮苷元比例的快速分析,从而为银杏叶最佳采收时机确定提供合理依据。
译  名：
Rapid Determination of Flavonol Glycosides in Folium Ginkgo by UPLC-UV
作  者：
Xiao Qiang;Chen Xiaoqiang;Zhou Dazhai;Key Laboratory of Biological Resources Protection and Utilization of Hubei Province,Hubei Institutes for Nationalities;
关键词：
UPLC-UV;;folium ginkgo leaf;;flavonol glycosides
摘  要：
In order to compare the contents of 3 main flavonol glycosides in the leaves of Ginkgo biloba L. ofdifferent species in the southwest of Hubei Province, the author carried out rapid determination of 3 mainflavonol glycosides in Folium Ginkgo in 5 min by UPLC-UV, and the calibration curve was linear in the rangeof 1-12 μg/m L, R2 was 0.9999 and RSD was less than 2.5%. This method is easy, rapid, accurate and suitablefor the quantitative analysis of flavonol glycosides. The results showed that among 5 species, flavonolglycosides content had obvious difference, the maximum content of flavonol glycosides was found in‘En G2'.Quercetin levels of all varieties were low while isorhamnetin had a higher proportion. It was inadequate todecide harvest time of ginkgo leaf only by total flavonoid aglycone content, rapid analysis of the proportion of 3main flavonoid aglycones in Ginkgo biloba L. could be achieved through the method in this paper, and it couldprovide reasonable basis for the determination of optimal harvest time of Ginkgo biloba.

相似文章

导出引用
计量
文章访问数: 16
HTML全文浏览量: 0
PDF下载量: 0

所属期刊

推荐期刊

友情链接Friendly link
农业信息研究所Agricultural information institute 农业智能知识服务平台Agricultural intelligent knowledge service platform 农业学术服务平台Agricultural Knowledge Service System 中国知网CNKI 维普网VIP 万方WANFANG DATA
联系我们Contact us

电话Tell：010-82109652-808

邮箱E-mail：agrijournal@caas.cn

服务Service
关于我们About us 意见建议Opinions and suggest
版权说明Copyright
单位：中国农业科学院农业信息研究所 中国农学会 © 2017中国农业科学院农业信息研究所 ICP备案号：京ICP备09089781号-46