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当前位置: 首页 > 文章 > QuEChERS-高效液相色谱法检测牛肉中的阿维菌素类药物残留 动物医学进展 2017,38 (11) 56-60
Position: Home > Articles > Determination of Avermectins Residues in Beef by QuEChERS-High Performance Liquid Chromatography Progress in Veterinary Medicine 2017,38 (11) 56-60

QuEChERS-高效液相色谱法检测牛肉中的阿维菌素类药物残留

作  者：
张伟;舒均喜;郑妍;李存
单  位：
天津农学院动物科学与动物医学学院
关键词：
阿维菌素类药物;QuEChERS;高效液相色谱;多残留检测
摘  要：
旨在建立牛肉组织中阿维菌素、伊维菌素、多拉菌素和甲氨基阿维菌素等4种阿维菌素类药物残留的QuEChERS-高效液相色谱检测方法。牛肉组织样品搅碎后,乙腈两次提取,QuEChERS净化,氮气吹干,含1-甲基咪唑和三氟乙酸酐的乙腈溶液衍生化,高效液相色谱-荧光检测器检测(激发波长365nm,发射波长475nm)。在0.1μg/mL~2μg/mL范围内4种药物均呈线性相关,相关系数大于0.999。检测限为2μg/kg(S/N=3),当添加浓度为2、10、40μg/kg时,4种药物的平均回收率在77.3%~97.2%,日内相对标准偏差为2.05%~4.91%(n=5),日间相对标准偏差为0.27%~1.64%(n=3)。建立的方法简便、灵敏、高效、安全,可用于动物组织中阿维菌素类药物残留的检测。
译  名：
Determination of Avermectins Residues in Beef by QuEChERS-High Performance Liquid Chromatography
作  者：
ZHANG Wei;SHU Jun-xi;ZHENG Yan;LI Cun;College of Animal Science and Veterinary Medicine,Tianjin Agricultural University;
单  位：
College of Animal Science and Veterinary Medicine,Tianjin Agricultural University
关键词：
avermectins;;QuEChERS;;high performance liquid chromatography;;multiresidue analysis
摘  要：
A multiresidue detection method was developed for the determination of abamectin,ivermectin,doramectin,emamectin benzoate in beef using QuEChERS-High performance liquid chromatography.The homogenized samples were extracted by acetonitrile twice,cleaned up by QuEChERS,and then dried by nitrogen.After being derivatised by 1-methylimidazole and trifluoroacetic anhydride in acetonitrile,the samples were determined by high performance chromatography with fluorescence detection(excitation wavelength is 365 nm,emission wavelength is 475 nm).In the range of 0.1μg/mL-2μg/mL,the calibration curves of abamectin,ivermectin,doramectin and emamectin benzoate showed good linearity,with the correlation coefficients being higher than 0.999.The limits of detection were 2μg/kg(S/N=3).At the spiked levels of 2,10,40μg/kg,the average recoveries were in the range of 77.3%-97.2%,with the intraday relative standard deviations ranging from 2.05 to 4.91%(n=5)and the daytime relative standard deviations ranging from 0.27 to 1.64%(n=3).The procedure provided a simple,sensitive,reliable and safe method for routine residual analysis.

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