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当前位置: 首页 > 文章 > 一株好氧反硝化细菌的分离鉴定及反硝化能力初测 天津农学院学报 2017 (2) 44-48
Position: Home > Articles > Identification and Denitrification Ability of an Aerobic Denitrifier Journal of Tianjin Agricultural University 2017 (2) 44-48

一株好氧反硝化细菌的分离鉴定及反硝化能力初测

作  者：
战海;王广军;陈成勋
单  位：
天津农学院水产学院;中国水产科学研究院珠江水产研究所
关键词：
好氧反硝化菌;反硝化能力;16S rRNA;不动杆菌属
摘  要：
采用BTB(溴甲基酚蓝)平板涂布分离法,从鳜鱼养殖池塘水体中分离出17株具有反硝化作用的细菌,通过初步筛选和反硝化能力的测定,挑选出一株具有较强反硝化能力的好氧反硝化细菌,命名为8F-3。菌株8F-3在24 h内将总氮从100.00 mg/L降至6.51 mg/L,去除率达到93.49%;将氨氮从50.000 mg/L降至1.966 mg/L,去除率为96.07%;将硝酸盐氮从50.00 mg/L降至3.51 mg/L,去除率为92.98%;将亚硝酸盐氮从0.096 mg/L降至0.071 mg/L,去除率为25.79%。该试验结果表明,菌株8F-3对氨氮和硝酸盐氮具有较强的去除能力,对亚硝酸盐氮也有一定去除作用,反硝化能力较强。经生理生化测试和16 SrRNA分子鉴定,初步鉴定该菌属于不动杆菌属(Acinetobacter)。
译  名：
Identification and Denitrification Ability of an Aerobic Denitrifier
作  者：
ZHAN Hai;WANG Guang-jun;CHEN Cheng-xun;Pearl River Fishery Research Institute, Chinese Academy of Fishery Sciences;College of Fisheries, Tianjin Agricultural University;
关键词：
aerobic denitrifier;;denitrification ability;;16S rRNA;;Acinetobacter
摘  要：
17 denitrification strains were isolated from mandarin fish pond water of Sanshui city using BTB(bromothymol blue) spread-plate technique, through the preliminary screening for denitrification strains and determination ability, one aerobic denitrifying bacteria which has strong determination, was named 8F-3. The results showed that the total content of nitrogen was reduced from 100.00 mg/L to 6.51 mg/L by 8F-3 strain in 24 hours and the rate of denitrification was 93.49%; Ammonia nitrogen was reduced from 50.000 mg/L to 1.966 mg/L, the removal rate was 96.07%, Nitrate nitrogen was reduced from 50.00 mg/L to 3.51 mg/L, the removal rate was 92.98%; Nitrite nitrogen was reduced from 0.096 mg/L to 0.071 mg/L, the removal rate was 25.79%. According to the blast results of 8F-3 strain 16 S r RNA sequence(Bootstrap 1 000 times)and physiological test for 8F-3 using API method, the bacteria was finally identified as Acinetobacter sp.

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