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当前位置: 首页 > 文章 > 甘薯病毒病害(SPVD)的分子生物学研究进展 核农学报 2020 (1) 62-70
Position: Home > Articles > 甘薯病毒病害(SPVD)的分子生物学研究进展 Journal of Nuclear Agricultural Sciences 2020 (1) 62-70

甘薯病毒病害(SPVD)的分子生物学研究进展

作  者：
孙钟毓;龚莺;赵琳;石江;毛碧增
关键词：
甘薯病毒病;甘薯羽状斑驳病毒;甘薯褪绿矮化病毒;沉默抑制蛋白;综合防控
摘  要：
甘薯病毒病害(SPVD)是最主要的甘薯病毒病害之一,研究表明甘薯羽状斑驳病毒(SPFMV)和甘薯褪绿矮化病毒(SPCSV)共同侵染形成SPVD.本文综述了SPFMV和SPCSV这2种病毒的生物学特性、检测方法、基因组结构与遗传变异等方面的研究进展,为阐明多种病毒混合侵染机制和防控措施提供了一定的理论依据.
作  者：
SHAO Shengzhi;NIE Jing;LIU Zhi;ZHANG Yongzhi;WANG Fang;Karyne M.ROGERS;YUAN Yuwei;Institute of Quality and Standards for Agricultural Products, Zhejiang Academy of Agricultural Science/Key Laboratory of Information Traceability for Agricultural Products, Ministry of Agriculture and Rural Affairs;National Isotope Centre, GNS Science;
关键词：
tea;;stable isotope;;processing method;;sample preparation
摘  要：
In order to investigate the effect of different processing technology on isotopic fractionation and different mesh sizes on the delta value of bulk stable isotopes in black and green tea sample, an analytical method on element analyzer-stable isotope ratio mass spectrometry(EA-IRMS) of tea for stable isotope ratio δ~(13)C, δ~(15)N, δ~2H, and δ~(18)O was established. A good precision of analytical method was achieved using two-or three-point calibration. No significant difference was observed for the isotopic fractionation of four isotope ratios between black and green tea processing using the same leaves, and no significant difference was concluded using different mesh sieves(no sieving, 80 mesh, 100 mesh, 120 mesh) on the effect of isotope ratios. Therefore, the tea-processing methods such as fermentation and sieving will not influence the isotopic fractionation results, which would provide a basis to establishment a tea data bank for the geographical traceability with different processing technology and for the pretreatment of plant sample before EA-IRMS.

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